Human IL-18/IL-1F4 Biotinylated Antibody

Catalog # Availability Size / Price Qty
D045-6
Product Details
Citations (9)
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Human IL-18/IL-1F4 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
This antibody reacts with human IL-18 on ELISA.
Source
Monoclonal Rat IgG2A Clone # 159-12B
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human IL-18/IL-1F4
Formulation
25 μg IgG in 50 μL volume of PBS containing 1% BSA and 0.09% sodium azide
Label
Biotin

Applications

Recommended Concentration
Sample

Human IL-18/IL-1F4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human IL‑18/IL‑1F4 Antibody (Catalog # D044-3)

Standard: Recombinant Human IL-18/IL-1F4 Protein (Catalog # B001-5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody solution is stable for one year from the date of purchase when stored at 4°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References
  1. McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
  2. Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
  3. Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
  4. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
  5. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
  6. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
  7. Okamura, H., et al. Nature 378, 88-91 (1995)
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

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Citations for Human IL-18/IL-1F4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j
    Authors: SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner
    J. Immunol., 2017-12-27;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  2. IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
    Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
    Respir. Res., 2017-08-22;18(1):159.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  3. Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
    Authors: LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton
    Cytokine, 2017-07-13;0(0):.
    Species: Human
    Sample Types: Tissue Culture Supernates
    Applications: ELISA Development (Detection)
  4. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
    Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
    J Immunol, 2015-07-31;195(5):2141-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  5. Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1.
    Authors: Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J
    Cytokine, 2013-11-23;65(2):222-30.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  6. Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
    Authors: Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
    Metab. Clin. Exp., 2009-07-01;58(7):946-53.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  7. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  8. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  9. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development

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