Human IL-23R Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB14001F-100
FAB14001F-025
Detection of IL‑23 R in Human PBMCs by Flow Cytometry.
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Product Details
Citations (5)
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Human IL-23R Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-23 R in direct ELISAs and Western blots. Does not cross-react with recombinant mouse IL‑23 R.
Source
Monoclonal Mouse IgG2B Clone # 218213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-23 R
Gly24-Ile354
Accession # Q5VWK5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-23 R antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑23 R in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 200 ng/mL LPS, 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), and 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) overnight to induce Th17 development were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human IL-23 R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB14001F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.

References
  1. Oppmann, B. et al. (2000) Immunity 13:715.
  2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
  3. Parham, C. et al. (2002) J. Immunol. 168:5448.
  4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
  5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name
Interleukin 23 Receptor
Entrez Gene IDs
149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)
Alternate Names
IBD17; IL-23 R; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor

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Citations for Human IL-23R Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Endogenous antisense RNA curbs CD39 expression in Crohn's disease
    Authors: RP Harshe, A Xie, M Vuerich, LA Frank, B Gromova, H Zhang, RJ Robles, S Mukherjee, E Csizmadia, E Kokkotou, AS Cheifetz, AC Moss, SK Kota, SC Robson, MS Longhi
    Nat Commun, 2020-11-18;11(1):5894.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Functional significance of MAIT cells in psoriatic arthritis
    Authors: SK Raychaudhu, C Abria, A Mitra, SP Raychaudhu
    Cytokine, 2019-09-18;125(0):154855.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The activation status of human macrophages presenting antigen determines the efficiency of Th17 responses.
    Authors: Arnold C, Gordon P, Barker R, Wilson H
    Immunobiology, 2015-01-01;220(1):10-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Boswellic acids reduce Th17 differentiation via blockade of IL-1beta-mediated IRAK1 signaling.
    Authors: Sturner K, Verse N, Yousef S, Martin R, Sospedra M
    Eur J Immunol, 2014-02-16;44(4):1200-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis.
    Authors: Bowness P, Ridley A, Shaw J
    J. Immunol., 2011-01-19;186(0):2672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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