Human IL-28 R alpha /IFN-lambda  R1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5260P
Detection of IL‑28 R alpha /IFN‑ lambda  R1 in Daudi Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
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Human IL-28 R alpha /IFN-lambda  R1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-28 R alpha /IFN-lambda R1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-28 alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 601106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
Arg21-Ala228
Accession # Q8IU57
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-28 Ra/IFN-? R1 antibody in Daudi Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of IL-28 Ra/IFN-? R1 antibody in PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑28 R alpha /IFN‑ lambda R1 in PBMCs by Flow Cytometry. Peripheral blood mononuclear cells (PBMCs) either untreated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human IL-28 Ra/IFN-lambda R1 PE-conjugated Monoclonal Antibody (Catalog # FAB5260P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-28 R alpha/IFN-lambda R1

IL-28 R alpha (IL-28 Receptor alpha subunit), also known as Interferon-lambda R1 (IFN-lambda R1) is a widely-expressed type I transmembrane glycoprotein that is Cytokine Receptor Family 2 member 12 (CRF2-12). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R. Its ligands, IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and IL-29 (IFN‑ lambda 1), are mainly produced by antigen presenting cells in response to viruses. The 520 amino acid (aa) mouse IL-28 R alpha contains a 208 aa extracellular domain (ECD) with a fibronectin type III motif. The human IL-28 R alpha ECD shares ~67% aa identity with rat and human IL‑28 R alpha. Isoforms of 491, 437, 322, 283, 244, 211 and 184 aa have alternate N- or C-termini, or lack transmembrane or internal sequences.

Long Name
Interleukin 28 Receptor alpha
Entrez Gene IDs
163702 (Human); 242700 (Mouse); 362628 (Rat)
Alternate Names
class II cytokine receptor CRF2/12; CRF2/12; CRF2-12; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; IFN-lambda R1; IFN-lambda receptor 1; IFN-lambda-R1; IFNLR; IFNLR1; IL-28 R alpha; IL-28 receptor subunit alpha; IL28R alpha; IL-28R1; IL28RA; IL-28Ra; IL-28R-alpha; Interferon lambda receptor 1; interferon lambda, receptor 1; interleukin 28 receptor A; interleukin 28 receptor, alpha (interferon, lambda receptor); interleukin 28 receptor, alpha; interleukin or cytokine receptor 2; interleukin-28 receptor subunit alpha; LICR2; Likely interleukin or cytokine receptor 2

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Citations for Human IL-28 R alpha /IFN-lambda  R1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Inhibition of Type III Interferon Expression in Intestinal Epithelial Cells-A Strategy Used by Coxsackie B Virus to Evade the Host's Innate Immune Response at the Primary Site of Infection?
    Authors: VM Stone, EE Ringqvist, PG Larsson, E Domsgen, U Holmlund, E Sverremark, M Flodström-
    Microorganisms, 2021-01-05;9(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Diminished secretion and function of IL-29 is associated with impaired IFN-? response of neonatal plasmacytoid dendritic cells
    Authors: L Wisgrill, I Wessely, A Netzl, L Pummer, K Sadeghi, A Spittler, A Berger, E Förster-Wa
    J. Leukoc. Biol., 2019-06-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Hepatitis B Virus e Antigen Activates the Suppressor of Cytokine Signaling 2 to Repress Interferon Action
    Authors: Y Yu, P Wan, Y Cao, W Zhang, J Chen, L Tan, Y Wang, Z Sun, Q Zhang, Y Wan, Y Zhu, F Liu, K Wu, Y Liu, J Wu
    Sci Rep, 2017-05-11;7(1):1729.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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