Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL‑28 R alpha /IFN‑ lambda R1 Monoclonal Antibody (Catalog # MAB5260, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Detection of IL‑28 R alpha /IFN‑ lambda R1 in PBMCs by Flow Cytometry. Peripheral blood mononuclear cells (PBMCs) either unstimulated (A) or treated (B) with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human IL‑28 R alpha /IFN‑ lambda R1 Monoclonal Antibody (Catalog # MAB5260) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-28 R alpha/IFN-lambda R1
IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a widely-expressed type I transmembrane glycoprotein that is cytokine receptor family 2 member 12 (CRF2-12). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R. Its ligands, IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and IL-29 (IFN‑ lambda 1), are mainly produced by antigen presenting cells in response to viruses. The 520 amino acid (aa) mouse IL-28 R alpha contains a 208 aa extracellular domain (ECD) with a fibronectin type III motif. The human IL-28 R alpha ECD shares ~67% aa identity with rat and human IL‑28 R alpha. Isoforms of 491, 437, 322, 283, 244, 211 and 184 aa have alternate N- or C-termini, or lack transmembrane or internal sequences.
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