IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL‑29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.
Human IL‑29/IFN‑ lambda 1 Biotinylated Antibody
R&D Systems | Catalog # BAF1598
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Detection, ELISA Development, ELISA Development (Detection)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑29/IFN‑ lambda 1
Gly20-Thr200
Accession # Q8IU54
Gly20-Thr200
Accession # Q8IU54
Specificity
Detects human IL‑29/IFN‑ lambda 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 100% cross-reactivity with recombinant human (rh) IL-28B is observed, 35% cross-reactivity with rhIL-28A is observed and less than 0.2% cross‑reactivity with rhIFN‑ alpha A, rhIFN-alpha B2, rhIFN‑ alpha G, rhIFN alpha I, and rhIFN-beta 1A is observed. In Western blots, approximately 25% cross-reactivity with rhIL-28A and rhIL‑28B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human IL‑29/IFN‑ lambda 1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human IL‑29/IFN-lambda 1 (Catalog # 1598-IL)
Sample: Recombinant Human IL‑29/IFN-lambda 1 (Catalog # 1598-IL)
IL-29/IFN-lambda 1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using BAF1598 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-29/IFN-lambda 1
References
- Vilcek, J. (2003) Nature Immunol. 4:8.
- Sheppard, P. et al. (2003) Nature Immunol. 4:63.
- Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
Long Name
Interleukin 29
Alternate Names
IFN-lambda 1, IL29
Entrez Gene IDs
282618 (Human)
Gene Symbol
IFNL1
UniProt
Additional IL-29/IFN-lambda 1 Products
Product Documents for Human IL‑29/IFN‑ lambda 1 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL‑29/IFN‑ lambda 1 Biotinylated Antibody
For research use only
Related Research Areas
Citations for Human IL‑29/IFN‑ lambda 1 Biotinylated Antibody
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Customer Reviews for Human IL‑29/IFN‑ lambda 1 Biotinylated Antibody (2)
4.5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: SerumSpecies: HumanVerified Customer | Posted 09/12/2022
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Application: ELISASample Tested: SerumSpecies: HumanVerified Customer | Posted 06/01/2018This antibody served as the detection in a sandwich ELISA with MAB15981 as the capture.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
