Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 373821
View all IL-32 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-32 alpha
Met1-Asn131
Accession # P24001

Specificity

Detects human IL-32 alpha and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 30% cross-reactivity with recombinant human (rh) IL-32 beta and 38% cross-reactivity with rhIL-32 gamma is detected.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human IL‑32 APC‑conjugated Antibody

Detection of IL-32 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑32 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL-32 APC-conjugated Monoclonal Antibody (Catalog # IC30402A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human IL‑32 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ Ionomycin and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-32

IL-32 alpha is one of at least nine potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4), which when full-length is 31 kDa in size. The predicted molecular weight of IL-32 alpha is approximately 15 kDa and it represents amino acids (aa) 1-18, 65-153, and 211-234 of the full-length (234 aa) form. Since the signal sequence of the ORF is aa 1-30, this isofom is like found intracellularly. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.

Long Name

Interleukin 32

Alternate Names

IL32, NK4, TAIF

Entrez Gene IDs

9235 (Human)

Gene Symbol

IL32

UniProt

P24001

Additional IL-32 Products

Product Documents for Human IL‑32 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑32 APC‑conjugated Antibody

For research use only

Citations for Human IL‑32 APC‑conjugated Antibody

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