Intracellular Staining by Flow Cytometry
|Detection of IL‑32 alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL‑32 alpha APC‑conjugated Monoclonal Antibody (Catalog # IC30402A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
IL-32 alpha is one of at least nine potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4), which when full-length is 31 kDa in size. The predicted molecular weight of IL-32 alpha is approximately 15 kDa and it represents amino acids (aa) 1-18, 65-153, and 211-234 of the full-length (234 aa) form. Since the signal sequence of the ORF is aa 1-30, this isofom is like found intracellularly. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN‑ gamma in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse.
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