Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 390412
View all IL-33 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-33
Ser112-Thr270
Accession # O95760

Specificity

Detects human IL-33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL‑33 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human IL‑33 Antibody

Detection of IL-33 by Western Blot

Detection of IL-33 by Western Blot

Over-expression and inhibition of miR-378a-3p modulate IL-33 expression. Stable expression of miR-378a-3p (Primir-378) or inhibition of miR-378a-3p (Inhibitor), and their respective controls (CTR PrimiR-378a, CTR Inhibitor) were obtained with Lentiviral vectors in HT-29 cells. (A) MiR-378a-3p Log2 fold change, and (B) IL-33 mRNA fold change relative to CTR cell lines by qPCR, (C) IL-33 protein and beta -actin by Western Blotting (WB) and bands quantification by densitometry analysis (Fiji Software) of Control Primir-378 and Primir-378 cell lines, and (D) Control Inhibitor and Inhibitor cell lines. Four experiments by duplicated were performed. Each cell line was compared with their respective control using Paired t-test. *P = < 0.05, **P = < 0.01, ***P = < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31824476), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-33 by Western Blot

Detection of IL-33 by Western Blot

Over-expression and inhibition of miR-378a-3p modulate IL-33 expression. Stable expression of miR-378a-3p (Primir-378) or inhibition of miR-378a-3p (Inhibitor), and their respective controls (CTR PrimiR-378a, CTR Inhibitor) were obtained with Lentiviral vectors in HT-29 cells. (A) MiR-378a-3p Log2 fold change, and (B) IL-33 mRNA fold change relative to CTR cell lines by qPCR, (C) IL-33 protein and beta -actin by Western Blotting (WB) and bands quantification by densitometry analysis (Fiji Software) of Control Primir-378 and Primir-378 cell lines, and (D) Control Inhibitor and Inhibitor cell lines. Four experiments by duplicated were performed. Each cell line was compared with their respective control using Paired t-test. *P = < 0.05, **P = < 0.01, ***P = < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31824476), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-33 by Western Blot

Detection of IL-33 by Western Blot

TNF alpha decreases miR-378a-3p and increases IL-33 expression in colonocytes. HT-29 cell line was stimulated with 10 ng/mL human recombinant TNF alpha (hr-TNF alpha ) for 3, 6, and 24 h. Fold change relative to non-stimulated control of (A) MiR-378a-3p and (B) IL-33 mRNA. (C) IL-33 protein was measured in HT-29 cells stimulated with hr-TNF alpha 10 ng/mL 12, 24, and 48 h by WB, (D) Band quantification of IL-33 protein normalized to beta -actin. (E) Fold change relative to non-stimulated control of PPARGC1B mRNA and (F) IL-8 mRNA levels (measured as control of TNF alpha stimulus). (G) AGO1 and (H) AGO2 mRNA levels were assessed as control of miRNA-biogenesis machinery. Four experiments by duplicated were analyzed using Two-way ANOVA with Bonferroni post-test. *P < 0.05, **P = < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31824476), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL‑33 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and Ca2+ ionomycin

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑33 (Catalog # 3625-IL)

Reviewed Applications

Read 1 review rated 4 using MAB3625 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-33

IL-33, also known as NF-HEV and DVS 27, is a  proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length 32 kDa IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal 18 kDa fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2, a receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2 with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL-33 share 52‑58% aa sequence identity with mouse and rat IL-33. Human IL-33 shares less than 20% aa sequence identity with other IL-1 family proteins.

References

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Entrez Gene IDs

90865 (Human); 77125 (Mouse)

Gene Symbol

IL33

UniProt

O95760

Additional IL-33 Products

Product Documents for Human IL‑33 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑33 Antibody

For research use only

Citations for Human IL‑33 Antibody

Customer Reviews for Human IL‑33 Antibody (1)

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  • Human IL-33 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with pAb (AF3625) and protein (3625-IL-010) to quantify IL-33 in human serum and plasma. This combination could not detect IL-33 in our samples but generated a good standard curve.
    Human IL‑33 Antibody MAB3625

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