Human IL-3R alpha/CD123 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: IL-3R alpha

IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).

Product Datasheets

Related Research Areas

• Acute Myeloid Leukemia
• Airway Inflammation and Remodeling
• B Cell Markers
• Basophils
• Cancer Stem Cell Markers
• Common beta Chain Receptor Family
• Cytokines and Receptors in Allergy and Asthma
• Eosinophils
• Hematopoietic Progenitor Cell Markers
• Myeloid Lineage Markers
• Plasmacytoid Dendritic Cells
• Receptors in the Jak/STAT Pathway
• T Cell Cytokine Signaling