Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
R&D Systems | Catalog # MAB10346
Recombinant Monoclonal Antibody For Research Use Only
Key Product Details
Species Reactivity
Human
Applications
Neutralization, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu137
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Product Specifications
Immunogen
Human IL-6R alpha
Specificity
Detects Human IL-6R alpha based on Sarilumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Sarilumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
Detection of IL-6R alpha in HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP by Flow Cytometry.
HEK293 Human cell line transfected with (A) Human IL-6R alpha or (B) irrelevant protein, and eGFP was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.
Detection of IL-6R alpha in U937 human histiocytic lymphoma cell line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.
IL-6 R alpha Enhancement of IL-6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6 R alpha Antibody.
Recombinant Human IL-6 Ra (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 Ra (100 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human IL-6 Ra (Research Grade Sarilumab) Monoclonal Antibody (Catalog # MAB10346). The ND50 is typically 0.04-0.2 µg/mL.Applications for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP and U937 human histiocytic lymphoma cell line
Sample: HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP and U937 human histiocytic lymphoma cell line
Neutralization
Measured by its ability to neutralize IL‑6 R alpha -mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 100 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-6R alpha
Long Name
Interleukin 6 Receptor alpha
Alternate Names
CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha
Gene Symbol
IL6R
Additional IL-6R alpha Products
Product Documents for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody
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Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?
A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.
