Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB10346-100
MAB10346-SP

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Detection of IL-6R alpha in HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP by Flow Cytometry.
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Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects Human IL-6R alpha based on Sarilumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Sarilumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu137
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human IL-6R alpha
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑6 R alpha -mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 100 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-6R alpha antibody in HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP antibody by Flow Cytometry. View Larger

Detection of IL-6R alpha in HEK293 Human cell line Transfected with Human IL-6R alpha and eGFP by Flow Cytometry. HEK293 Human cell line transfected with (A) Human IL-6R alpha or (B) irrelevant protein, and eGFP was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of IL-6R alpha antibody in U937 human histiocytic lymphoma cell line antibody by Flow Cytometry. View Larger

Detection of IL-6R alpha in U937 human histiocytic lymphoma cell line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Human Anti-Human IL-6R alpha (Research Grade Sarilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10346, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Neutralization IL-6 R alpha  Enhancement of IL-6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6 R alpha  Antibody. View Larger

IL-6 R alpha Enhancement of IL-6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6 R alpha Antibody. Recombinant Human IL-6 Ra (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6 Ra (100 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human IL-6 Ra (Research Grade Sarilumab) Monoclonal Antibody (Catalog # MAB10346). The ND50 is typically 0.04-0.2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

Sarilumab is a biosimilar directed against human IL-6R alpha.

Long Name
Interleukin 6 Receptor alpha
Entrez Gene IDs
3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

Product Datasheets

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FAQs

  1. Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    • The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

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