Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Integrin alpha 3/CD49c
(Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily,
of cell membrane adhesion molecules (1-3). Integrins are
nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha -
and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The
alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only
associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to
generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain
and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy
chain contains seven 60 aa repeats that fold into a propeller-like
structure (7). Sequences involving the first three repeats are
associated with ligand binding (1). The light chain has two cytoplasmic
alternate splice forms. The A form cytoplasmic domain is 52 aa, while
the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9,
CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it
to bind to additional factors (8).
Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
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