Human Integrin alpha 3/CD49c Alexa Fluor® 750-conjugated Antibody

FAB1345S Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB1345S-100UG

Bulk Orders

Human Integrin alpha 3/CD49c Alexa Fluor® 750-conjugated Antibody in Flow Cytometry

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha 3/CD49c Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 3/CD49c.

Source

Monoclonal Mouse IgG₁ Clone # IA3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human milk epithelial cell line

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 750

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

View Larger

Detection of Integrin alpha 3/CD49c in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 3/CD49c Alexa Fluor® 750‑conjugated Monoclonal Antibody (Catalog # FAB1345S, filled histogram) or isotype control antibody (Catalog # IC002S, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 3/CD49c

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).

References

Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Entrez Gene IDs

3675 (Human); 16400 (Mouse)

Alternate Names

antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; Integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a