Human Integrin  alpha 3/CD49c Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha 3/CD49c.
  • Source
    Monoclonal Mouse IgG1 Clone # IA3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human milk epithelial cell line
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    HT1080 human fibrosarcoma cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Integrin  alpha 3/CD49c in HT1080 Human Cell Line. Integrin  alpha 3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin  alpha 3/CD49c Monoclonal Antibody (Catalog # MAB1345) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 3/CD49c
VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).
  • References:
    1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
    2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
    3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
    4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
    5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
    6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
    7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
    8. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.
  • Entrez Gene IDs:
    3675 (Human); 16400 (Mouse)
  • Alternate Names:
    antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; Integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands
    Authors: TL Tremblay, JJ Hill
    Sci Rep, 2017;7(0):46574.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  2. Keratins Stabilize Hemidesmosomes through Regulation of beta4-Integrin Turnover.
    Authors: Seltmann K, Cheng F, Wiche G, Eriksson J, Magin T
    J Invest Dermatol, 2015;135(6):1609-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study.
    Authors: Turner NJ, Murphy MO, Kielty CM
    Circulation, 2006;114(8):820-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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