Human Integrin  alpha 3/CD49c Antibody

Catalog # Availability Size / Price Qty
MAB1345
MAB1345-SP

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Detection of Integrin alpha 3/CD49c in HT1080 Human Cell Line by Flow Cytometry.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)

Human Integrin  alpha 3/CD49c Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 3/CD49c.
Source
Monoclonal Mouse IgG1 Clone # IA3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human milk epithelial cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin alpha 3/CD49c antibody in HT1080 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 3/CD49c in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 3/CD49c Monoclonal Antibody (Catalog # MAB1345, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Integrin a3/CD49c antibody in HT1080 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha 3/CD49c in HT1080 Human Cell Line. Integrin a3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin a3/CD49c Monoclonal Antibody (Catalog # MAB1345) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 3/CD49c

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).

References
  1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
  4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
  5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
  6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
  7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
  8. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.
Entrez Gene IDs
3675 (Human); 16400 (Mouse)
Alternate Names
antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; Integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a

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Citations for Human Integrin  alpha 3/CD49c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. 3D bioprinting of an implantable xeno‐free vascularized human skin graft
    Authors: Tania Baltazar, Bo Jiang, Alejandra Moncayo, Jonathan Merola, Mohammad Z. Albanna, W. Mark Saltzman et al.
    Bioengineering & Translational Medicine
  2. An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family
    Authors: C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse
    PLoS ONE, 2022-09-15;17(9):e0274667.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  3. Essential Functions of Glycans in Human Epithelia Dissected by a CRISPR-Cas9-Engineered Human Organotypic Skin Model
    Authors: Sally Dabelsteen, Emil M.H. Pallesen, Irina N. Marinova, Mathias I. Nielsen, Maria Adamopoulou, Troels B. Rømer et al.
    Developmental Cell
  4. Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands
    Authors: TL Tremblay, JJ Hill
    Sci Rep, 2017-04-19;7(0):46574.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  5. Keratins Stabilize Hemidesmosomes through Regulation of beta4-Integrin Turnover.
    Authors: Seltmann K, Cheng F, Wiche G, Eriksson J, Magin T
    J Invest Dermatol, 2015-02-10;135(6):1609-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study.
    Authors: Turner NJ, Murphy MO, Kielty CM
    Circulation, 2006-08-14;114(8):820-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human Integrin  alpha 3/CD49c Antibody

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Human Integrin  alpha 3/CD49c Antibody
By Anonymous on 12/06/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human Integrin  alpha 3/CD49c Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human