Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # IA3
View all Integrin alpha 3/CD49c Primary Antibodies »

Product Specifications

Immunogen

Human milk epithelial cell line

Specificity

Detects human Integrin alpha 3/CD49c.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha 3/CD49c Fluorescein‑conjugated Antibody

Detection of Integrin a3/CD49c antibody in HT1080 Human Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha 3/CD49c in HT1080 Human Cell Line by Flow Cytometry.

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin a3/CD49c Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1345F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alpha 3/CD49c Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HT1080 human fibrosarcoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 3/CD49c

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1 - 3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130 - 150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8).

References

  1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
  4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
  5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
  6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
  7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
  8. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Alternate Names

CD49c, ITGA3

Entrez Gene IDs

3675 (Human); 16400 (Mouse)

Gene Symbol

ITGA3

Additional Integrin alpha 3/CD49c Products

Product Documents for Human Integrin  alpha 3/CD49c Fluorescein‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human Integrin  alpha 3/CD49c Fluorescein‑conjugated Antibody

For research use only

Citations for Human Integrin  alpha 3/CD49c Fluorescein‑conjugated Antibody

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