Detection of Integrin alpha 3/CD49c in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 3/CD49c Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1345F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 3/CD49c
(Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily,
of cell membrane adhesion molecules (1 - 3). Integrins are
nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha -
and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The
alpha 3/CD49c subunit is a 130 - 150 kDa type I TM glycoprotein. It only
associates with the beta 1 integrin subunit. It is synthesized as a 1051
amino acid (aa) precursor that undergoes proteolytic cleavage to
generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain
and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy
chain contains seven 60 aa repeats that fold into a propeller-like
structure (7). Sequences involving the first three repeats are
associated with ligand binding (1). The light chain has two cytoplasmic
alternate splice forms. The A form cytoplasmic domain is 52 aa, while
the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88%
aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and
laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9,
CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it
to bind to additional factors (8).
Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.