Human Integrin  alpha 3/CD49c Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1345
Integrin  alpha 3/CD49c in HT1080 Human Cell Line.
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Citations (1)
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Human Integrin  alpha 3/CD49c Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 3/CD49c.
Source
Monoclonal Mouse IgG1 Clone # IA3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human milk epithelial cell line
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HT1080 human fibrosarcoma cell line
Immunocytochemistry
8-25 µg/mL
Immersion fixed HT1080 human fibrosarcoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin a3/CD49c antibody in HT1080 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha 3/CD49c in HT1080 Human Cell Line. Integrin a3/CD49c was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin a3/CD49c Biotinylated Monoclonal Antibody (Catalog # BAM1345) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 3/CD49c

The alpha 3 subunit, also known as CD49c and VLA-3 alpha subunit, forms a non-covalent heterodimer with the Integrin beta 1 subunit (CD29). Integrin alpha 3 beta 1 is a receptor for laminin, fibronectin, collagen, epiligrin, and thrombospondin.

Entrez Gene IDs
3675 (Human); 16400 (Mouse)
Alternate Names
antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; Integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a

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Citation for Human Integrin  alpha 3/CD49c Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Glycosylation modulates melanoma cell alpha2beta1 and alpha3beta1 integrin interactions with type IV collagen.
    Authors: Stawikowski M, Aukszi B, Stawikowska R, Cudic M, Fields G
    J Biol Chem, 2014-06-23;289(31):21591-604.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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