Human Integrin alpha 5/CD49e Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1864G-100UG

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Detection of Integrin  alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry.
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Human Integrin alpha 5/CD49e Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 5/CD49e.
Source
Monoclonal Mouse IgG1 Clone # 238307
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e
Phe42-Tyr995
Accession # P08648
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

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Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Integrin  alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry. View Larger

Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin a5/CD49e Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1864G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 5/CD49e

The Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

References
  1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
  2. Te Velde, A. et al. (1988) J. Immunol. 140:1548.
Entrez Gene IDs
3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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