Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 238307
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e
Phe42-Tyr995
Accession # P08648

Specificity

Detects human Integrin alpha 5/CD49e.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha 5/CD49e APC‑conjugated Antibody

Detection of Integrin a5/CD49e antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin a5/CD49e APC-conjugated Monoclonal Antibody (Catalog # FAB1864A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alpha 5/CD49e APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 5/CD49e

The Integrin alpha 5 subunit, also known as CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

References

  1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
  2. Te Velde, A. et al. (1988) J. Immunol. 140:1548.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

Additional Integrin alpha 5/CD49e Products

Product Documents for Human Integrin  alpha 5/CD49e APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Integrin  alpha 5/CD49e APC‑conjugated Antibody

For research use only

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