Human Integrin alpha 5/CD49e APC-conjugated Antibody

Catalog #: FAB1864A Datasheet / COA / SDS

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FAB1864A

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Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry.

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Human Integrin alpha 5/CD49e APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 5/CD49e.

Source

Monoclonal Mouse IgG₁ Clone # 238307

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e
Phe42-Tyr995
Accession # P08648

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin a5/CD49e APC-conjugated Monoclonal Antibody (Catalog # FAB1864A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 5/CD49e

The Integrin alpha 5 subunit, also known as CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

References

Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Te Velde, A. et al. (1988) J. Immunol. 140:1548.

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Alternate Names

CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5