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Human Integrin  alpha 5/CD49e Antibody

Catalog #: MAB1864
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1864
MAB1864-SP

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Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  alpha 5/CD49e Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 5/CD49e.
Source
Monoclonal Mouse IgG1 Clone # 238307
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e
Phe42-Tyr995
Accession # P08648
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Adhesion Activation
The adhesion of K562 human chronic myelogenous leukemia cells (5 x 104 cells/well) to immobilized Bovine Fibronectin (Catalog # 1030-FN, 10 µg/mL, 100 µL/well) was maximally enhanced (2-2.5 fold) by pre-treatment of cells with 500 ng/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
Immersion fixed IMR‑90 human lung fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin alpha 5/CD49e antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin alpha 5/CD49e Monoclonal Antibody (Catalog # MAB1864, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry View Larger

Integrin  alpha 5/CD49e in IMR‑90 Human Cell Line. Integrin  alpha 5/CD49e was detected in immersion fixed IMR‑90 human lung fibroblast cell line using Mouse Anti-Human Integrin  alpha 5/CD49e Monoclonal Antibody (Catalog # MAB1864) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

The Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

References
  1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
  2. Te Velde, A. et al. (1988) J. Immunol. 140:1548.
Entrez Gene IDs
3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

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Citations for Human Integrin  alpha 5/CD49e Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. 3D motion of vesicles along microtubules helps them to circumvent obstacles in cells.
    Authors: Verdeny-Vilanova I, Wehnekamp F, Mohan N et al.
    J. Cell. Sci.
  2. Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay
    Authors: MG Jeong, K Zhou, S Park, H An, Y Kwon, Y Chang, DH Kim, SH Ryu
    Experimental & Molecular Medicine, 2021-02-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Promoter Methylation Leads to Decreased ZFP36 Expression and Deregulated NLRP3 Inflammasome Activation in Psoriatic Fibroblasts
    Authors: Matteo Bertesi, Sebastian Fantini, Claudia Alecci, Roberta Lotti, Andrea Martello, Sandra Parenti et al.
    Frontiers in Medicine
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.
    Authors: Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P
    Biomaterials, 2009-04-10;30(20):3415-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Dentin matrix protein-1 isoforms promote differential cell attachment and migration.
    Authors: Von Marschall Z, Fisher LW
    J. Biol. Chem., 2008-09-25;283(47):32730-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels.
    Authors: Chapple SJ, Keeley TP, Mastronicola D et al.
    Free Radic. Biol. Med.

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