Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Sheep IgG
View all Integrin alpha 1/CD49a Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 1/CD49a
Phe29-Pro1141
Accession # P56199

Specificity

Detects human Integrin  alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin  alpha 2, rhIntegrin  alpha 11, recombinant mouse (rm) Integrin  alpha 2, and rmIntegrin  alpha 11 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Integrin  alpha 1/CD49a APC‑conjugated Antibody

Detection of Integrin a1/CD49a antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha 1/CD49a in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Integrin a1/CD49a APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5676A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alpha 1/CD49a APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha 1/CD49a

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190‑210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin  alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I‑domain (aa 147‑360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765‑1179. Over aa 29‑1141 (the ECD), human Integrin  alpha 1 shares 88% aa identity with mouse Integrin  alpha 1.

Alternate Names

CD49a, ITGA1, VLA-1

Entrez Gene IDs

3672 (Human); 109700 (Mouse); 25118 (Rat)

Gene Symbol

ITGA1

UniProt

P56199

Additional Integrin alpha 1/CD49a Products

Product Documents for Human Integrin  alpha 1/CD49a APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  alpha 1/CD49a APC‑conjugated Antibody

For research use only

Citations for Human Integrin  alpha 1/CD49a APC‑conjugated Antibody

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