Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Integrin alpha 1/CD49a Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin  alpha 1/CD49a
Phe29-Pro1141
Accession # P56199

Specificity

Detects human Integrin  alpha 1/CD49a in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Integrin  alpha 11, recombinant mouse (rm) Integrin alpha 11, rhIntegrin  alpha 2, and rmIntegrin  alpha 2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Integrin  alpha 1/CD49a Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Integrin  alpha 1/CD49a

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 1/CD49a

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190‑210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin  alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I‑domain (aa 147‑360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765‑1179. Over aa 29‑1141 (the ECD), human Integrin  alpha 1 shares 88% aa identity with mouse Integrin  alpha 1.

Alternate Names

CD49a, ITGA1, VLA-1

Entrez Gene IDs

3672 (Human); 109700 (Mouse); 25118 (Rat)

Gene Symbol

ITGA1

UniProt

P56199

Additional Integrin alpha 1/CD49a Products

Product Documents for Human Integrin  alpha 1/CD49a Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  alpha 1/CD49a Biotinylated Antibody

For research use only

Citations for Human Integrin  alpha 1/CD49a Biotinylated Antibody

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