Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Integrin alpha 1/CD49a Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 1/CD49a
Phe29-Pro1141
Accession # P56199

Specificity

Detects human Integrin  alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin  alpha 2, rhIntegrin  alpha 11, recombinant mouse (rm) Integrin  alpha 2, and rmIntegrin  alpha 11 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Integrin  alpha 1/CD49a Antibody

Detection of Human Integrin a 1/CD49a antibody by Western Blot.

Detection of Human Integrin alpha 1/CD49a by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti- Human Integrin a1/CD49a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5676) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin a 1/CD49a at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Integrin a1/CD49a antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha 1/CD49a in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Integrin a1/CD49a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5676, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
Integrin a1/CD49a antibody in Human Stomach Cancer Tissue by Immunohistochemistry (IHC-P).

Integrin alpha 1/CD49a in Human Stomach Cancer Tissue.

Integrin a1/CD49a was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Sheep Anti-Human Integrin a1/CD49a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5676) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Integrin  alpha 1/CD49a Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line

Reviewed Applications

Read 3 reviews rated 5 using AF5676 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 1/CD49a

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190‑210 kDa member of the integrin alpha chain family of molecules. It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium. Integrin  alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI. It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus. Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail. The ECD contains one vWFA/I‑domain (aa 147‑360) that binds collagen, plus multiple divalent cation binding sites. Potential splice variants exist that show a two and four aa substitution for aa 765‑1179. Over aa 29‑1141 (the ECD), human Integrin  alpha 1 shares 88% aa identity with mouse Integrin  alpha 1.

Alternate Names

CD49a, ITGA1, VLA-1

Entrez Gene IDs

3672 (Human); 109700 (Mouse); 25118 (Rat)

Gene Symbol

ITGA1

UniProt

P56199

Additional Integrin alpha 1/CD49a Products

Product Documents for Human Integrin  alpha 1/CD49a Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  alpha 1/CD49a Antibody

For research use only

Citations for Human Integrin  alpha 1/CD49a Antibody

Customer Reviews for Human Integrin  alpha 1/CD49a Antibody (3)

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Showing  1 - 3 of 3 reviews Showing All
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  • Human Integrin  alpha 1/CD49a Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 01/21/2020
    IHC-paraffin working dilution: 1ug/mL with Bond Refine polymer Detection rabbit anti-goat IgG as a Linker antibody epitope retrieval: basic (BOND ER2)
    Human Integrin  alpha 1/CD49a Antibody AF5676
  • Human Integrin  alpha 1/CD49a Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Placental tissue
    Species: Human
    Verified Customer | Posted 01/21/2020
    IHC-paraffin working dilution: 1ug/mL with Bond Refine polymer Detection rabbit anti-goat IgG as a Linker antibody epitope retrieval: basic (BOND ER2)
    Human Integrin  alpha 1/CD49a Antibody AF5676
  • Human Integrin  alpha 1/CD49a Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Intestine
    Species: Human
    Verified Customer | Posted 01/21/2020
    working dilution: 1ug/mL with Bond Refine polymer Detection rabbit anti-goat IgG as a Linker antibody
    Human Integrin  alpha 1/CD49a Antibody AF5676

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Protocols

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