The alpha 5 subunit, also known as CD49e and VLA-5 alpha subunit, forms a heterodimer with the Integrin beta 1 subunit (CD29). Integrin alpha 5 beta 1 is a receptor for fibronectin and neural adhesion molecule L1.
Human Integrin alpha 5/CD49e Biotinylated Antibody
R&D Systems | Catalog # BAF1864
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e
Phe42-Tyr995
Accession # P08648
Phe42-Tyr995
Accession # P08648
Specificity
Detects human Integrin alpha 5/CD49e in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin alpha 5 is observed and less than 2% cross-reactivity with recombinant human Integrin alpha M, rmIntegrin alpha 2, rmIntegrin alpha 4, and rmIntegrin alpha E is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Integrin alpha 5/CD49e Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Integrin alpha 5/CD49e
Sample: Recombinant Human Integrin alpha 5/CD49e
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Integrin alpha 5/CD49e
Alternate Names
CD49e, ITGA5, VLA-5 alpha
Gene Symbol
ITGA5
UniProt
Additional Integrin alpha 5/CD49e Products
Product Documents for Human Integrin alpha 5/CD49e Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Integrin alpha 5/CD49e Biotinylated Antibody
For research use only
Citations for Human Integrin alpha 5/CD49e Biotinylated Antibody
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