Human Integrin alpha 5/CD49e Antibody Summary
Accession # P08648
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Integrin a5/CD49e by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a5/CD49e at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Integrin alpha 5/CD49e by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 5/CD49e at approximately 165 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Integrin alpha 5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 5/CD49e
The alpha 5 subunit, also known as CD49e and VLA-5 alpha subunit, forms a heterodimer with the Integrin beta 1 subunit (CD29). Integrin alpha 5 beta 1 is a receptor for fibronectin and neural adhesion molecule L1.
Citations for Human Integrin alpha 5/CD49e Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Induction of cyclophilin A by influenza A virus infection facilitates group A Streptococcus coinfection
Authors: X Bai, W Yang, X Luan, H Li, H Li, D Tian, W Fan, J Li, B Wang, W Liu, L Sun
Cell Reports, 2021;35(7):109159.
Sample Types: Whole Cells
Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics
Authors: J Wiener, D Kokotek, S Rosowski, H Lickert, M Meier
Sci Rep, 2020;10(1):1457.
Sample Types: Cell Culture Lysates
Applications: ELISA Capture
Macrophage-secreted factors promote a profibrotic phenotype in human preadipocytes.
Authors: Keophiphath M, Achard V, Henegar C, Rouault C, Clement K, Lacasa D
Mol. Endocrinol., 2009;23(1):11-24.
Sample Types: Whole Cells
Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.
Authors: Page JL, Johnson MC, Olsavsky KM
Toxicol. Sci., 2007;97(2):384-97.
Sample Types: Cell Lysates
Applications: Western Blot
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