Human Integrin alpha 5/CD49e Antibody

R&D Systems | Catalog # AF1864

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Adhesion Blockade, Flow Cytometry, Simple Western, CyTOF-ready

Cited:

Western Blot, Neutralization, Immunocytochemistry, Co-Immunoprecipitation, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Integrin alpha 5/CD49e Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Integrin  alpha 5/CD49e
Phe42-Tyr995
Accession # P08648

Specificity

Detects human Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse (rm) Integrin  alpha 5 and less than 5% cross-reactivity with recombinant human Integrin  alpha M, rmIntegrin  alpha 2, rmIntegrin  alpha 4, and rmIntegrin  alpha E is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Integrin alpha 5/CD49e Antibody

Detection of Human Integrin a5/CD49e antibody by Western Blot.

Detection of Human Integrin a5/CD49e by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a5/CD49e at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human Integrin  alpha 5/CD49e by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (U87 MG) (NBP2-49844), Exosome Standards (PC‑3) (NBP2-49856) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin  alpha 5/CD49e at approximately 165 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Integrin  alpha 5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Detection of Human Integrin  alpha 5/CD49e by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha 5/CD49e at approximately 165 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Integrin  alpha 5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Integrin alpha 5/CD49e Antibody

Application
Recommended Usage

Adhesion Blockade

The adhesion of K562 human chronic myelogenous leukemia cells (5 x 104 cells/well) to immobilized bovine Fibronectin (Catalog # 1030-FN, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Simple Western

20 µg/mL
Sample: Exosome Standard (U87 MG) (Catalog # NBP2-49844), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Reviewed Applications

Read 2 reviews rated 4.5 using AF1864 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 5/CD49e

The alpha 5 subunit, also known as CD49e and VLA-5 alpha subunit, forms a heterodimer with the Integrin beta 1 subunit (CD29). Integrin alpha 5 beta 1 is a receptor for fibronectin and neural adhesion molecule L1.

Alternate Names

CD49e, ITGA5, VLA-5 alpha

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Gene Symbol

ITGA5

UniProt

P08648

Additional Integrin alpha 5/CD49e Products

Product Documents for Human Integrin alpha 5/CD49e Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Integrin alpha 5/CD49e Antibody

For research use only

Citations for Human Integrin alpha 5/CD49e Antibody

Customer Reviews for Human Integrin alpha 5/CD49e Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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  • Human Integrin  alpha 5/CD49e Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HMVEC human microvascular endothelial cells
    Species: Rabbit
    Verified Customer | Posted 04/25/2018
  • Human Integrin  alpha 5/CD49e Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: blood endothelial cells
    Species: Human
    Verified Customer | Posted 04/24/2018
    Used at concentration of 1 in 2000 Band seen at around 160kDa Worked in both reducing and non-reducing conditions Would use again
    Human Integrin alpha 5/CD49e Antibody AF1864

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