Detection of Integrin alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry.
HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha V/CD51 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1219G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V/CD51
Integrin alpha V (CD51), also known as Vitronectin Receptor subunit alpha, is a 140-150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human alpha V is a 1018 amino acid (aa) type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta -propeller domain (aa 46-483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alpha V is 92% aa identical to mouse alpha V ECD.
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