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Human Integrin  alpha V/CD51 Alexa Fluor® 488-conjugated Antibody

    
Datasheet / CoA / SDS
<P align=left>Detection of Integrin&nbsp; alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry.

Flow Cytometry

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  • Species Reactivity
    Human
  • Specificity
    Detects the ectodomain of human Integrin alpha V/CD51.
  • Source
    Monoclonal Mouse IgG1 Clone # P2W7
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Ocular melanoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry Integrin alpha V/CD51 Antibody (P2W7) [Alexa Fluor® 488]
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Detection of Integrin  alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin  alpha V/CD51 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1219G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V/CD51

Integrin alpha V (CD51), also known as Vitronectin Receptor subunit alpha, is a 140-150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human  alpha V is a 1018 amino acid (aa) type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta -propeller domain (aa 46-483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alpha V is 92% aa identical to mouse alpha V ECD.

  • Entrez Gene IDs:
    3685 (Human); 16410 (Mouse); 296456 (Rat)
  • Alternate Names:
    antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142
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FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

 Ctrl IC002G 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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