Human Integrin  alpha V/CD51 PE-conjugated Antibody

(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects the ectodomain of human Integrin alpha V/CD51.
  • Source
    Monoclonal Mouse IgG1 Clone # P2W7
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Ocular melanoma cell line
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry.

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin  alpha V/CD51 PE-conjugated Monoclonal Antibody (Catalog # FAB1219P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V/CD51

Integrin alpha V (CD51; also vitronectin receptor subunit alpha) is a 140‑150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human  alpha V is a 1018 aa type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta ‑propellor domain (aa 46-483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alpha V is 92% aa identical to mouse alpha V ECD.

  • Entrez Gene IDs:
    3685 (Human); 16410 (Mouse); 296456 (Rat)
  • Alternate Names:
    antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 2 reviews)

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Read what people are saying who have used Human Integrin  alpha V/CD51 PE-conjugated Antibody.

We have 2 reviews tested in 1 species: Human.
We have 2 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human Integrin  alpha V/CD51 PE-conjugated Antibody FAB1219P
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Excellent
 Flow Human Anonymous 04/10/2017
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Image Details
Flow Cytometry Human Integrin  alpha V/CD51 PE-conjugated Antibody FAB1219P
Flow Cytometry: Human Integrin  alpha V/CD51 PE-conjugated Antibody [FAB1219P]

Summary

ApplicationFlow Cytometry
Sample Tested293T human embryonic kidney cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsNegative is integrin av knockout cells
Flow Cytometry Human Integrin  alpha V/CD51 PE-conjugated Antibody FAB1219P
Enlarge
Excellent
 Flow Human Anonymous 05/26/2016
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Image Details
Flow Cytometry Human Integrin  alpha V/CD51 PE-conjugated Antibody FAB1219P
Flow Cytometry: Human Integrin  alpha V/CD51 PE-conjugated Antibody [FAB1219P]

Summary

ApplicationFlow Cytometry
Sample TestedHEK293 human embryonic kidney cell line
SpeciesHuman

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