Human Integrin  alpha V/CD51 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1219P
Human Integrin  alpha V/CD51 PE-conjugated Antibody in Data
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Human Integrin  alpha V/CD51 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects the ectodomain of human Integrin alpha V/CD51.
Source
Monoclonal Mouse IgG1 Clone # P2W7
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Ocular melanoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Integrin  alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry. View Larger

Detection of Integrin alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 PE-conjugated Monoclonal Antibody (Catalog # FAB1219P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha V/CD51

Integrin alpha V (CD51; also vitronectin receptor subunit alpha) is a 140‑150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human  alpha V is a 1018 aa type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta ‑propellor domain (aa 46-483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alpha V is 92% aa identical to mouse alpha V ECD.

Entrez Gene IDs
3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142

Product Datasheets

FAQs

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Reviews for Human Integrin  alpha V/CD51 PE-conjugated Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human Integrin  alpha V/CD51 PE-conjugated Antibody
By Anonymous on 04/10/2017
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Human

Negative is integrin av knockout cells


Human Integrin  alpha V/CD51 PE-conjugated Antibody
By Anonymous on 05/26/2016
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human