Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # P2W7
View all Integrin alpha V/CD51 Primary Antibodies »

Product Specifications

Immunogen

Ocular melanoma cell line

Specificity

Detects the ectodomain of human Integrin alpha V/CD51.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha V/CD51 PE‑conjugated Antibody

Detection of Integrin aV/CD51 antibody in HT1080 Human Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry.

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin aV/CD51 PE-conjugated Monoclonal Antibody (Catalog # FAB1219P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin  alpha V/CD51 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HT1080 human fibrosarcoma cell line

Reviewed Applications

Read 2 reviews rated 5 using FAB1219P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha V/CD51

Integrin alpha V (CD51; also vitronectin receptor subunit alpha) is a 140‑150 kDa member of the integrin alpha -chain family of adhesion molecules. It forms disulfide-linked integral membrane heterodimers with at least five beta -chains, including beta 1, 3, 5, 6 and 8. Human  alpha V is a 1018 aa type I transmembrane glycoprotein that contains a 962 aa extracellular domain (ECD) (aa 31-992), and a short 32 aa cytoplasmic tail. The ECD contains seven FG (PheAlaGly)-GAP (GlyAlaPro) repeats that form a beta ‑propellor domain (aa 46-483). Furin cleavage of the alpha V ECD occurs after Gly889, generating a disulfide-linked heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2 and TSP. The ECD of human alpha V is 92% aa identical to mouse alpha V ECD.

Alternate Names

CD51, ITGAV, MSK8, Vitronectin Receptor alpha, VNRA

Entrez Gene IDs

3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)

Gene Symbol

ITGAV

Additional Integrin alpha V/CD51 Products

Product Documents for Human Integrin  alpha V/CD51 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  alpha V/CD51 PE‑conjugated Antibody

For research use only

Citations for Human Integrin  alpha V/CD51 PE‑conjugated Antibody

Customer Reviews for Human Integrin  alpha V/CD51 PE‑conjugated Antibody (2)

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  • Human Integrin  alpha V/CD51 PE-conjugated Antibody
    Name: Xianming Zhang
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 04/10/2017
    Negative is integrin av knockout cells
    Human Integrin  alpha V/CD51 PE‑conjugated Antibody FAB1219P
  • Human Integrin  alpha V/CD51 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 05/26/2016
    Human Integrin  alpha V/CD51 PE‑conjugated Antibody FAB1219P

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