Human Integrin  alpha V/CD51 Antibody

• Species Reactivity
  Human
• Specificity
  Detects the ectodomain of human Integrin alpha V/CD51.
• Source
  Monoclonal Mouse IgG₁ Clone # P2W7
• Purification
  Protein A or G purified from hybridoma culture supernatant
• Immunogen
  Ocular melanoma cell line
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Flow Cytometry
  0.25 µg/10⁶ cells
  See below
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
• Immunocytochemistry
  8-25 µg/mL
  See below

Flow Cytometry click image to view larger

Detection of Integrin  alpha V/CD51 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin  alpha V/CD51 Monoclonal Antibody (Catalog # MAB1219, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B).

Immunocytochemistry click image to view larger

Integrin  alpha V/CD51 in HT1080 Human Cell Line. Integrin  alpha V/CD51 was detected in immersion fixed HT1080 human fibrosarcoma cell line using 10 µg/mL Mouse Anti-Human Integrin  alpha V/CD51 Monoclonal Antibody (Catalog # MAB1219) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reconstitution
  Reconstitute at 0.5 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Entrez Gene IDs:
  3685 (Human); 16410 (Mouse); 296456 (Rat)

• Alternate Names:
  antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142

FAQs

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