Human Integrin  alpha V/CD51 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1219
Human Integrin  alpha V/CD51 Biotinylated Antibody in Data
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Human Integrin  alpha V/CD51 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha V/CD51 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Integrin  alpha V is observed, 5% cross‑reactivity with recombinant human (rh) Integrin alpha 2 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5 and rmIntegrin alpha E is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51
Phe31-Val992
Accession # NP_002201
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Integrin  alpha V/CD51
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Integrin  alpha V/CD51 in Human Mesenchymal Stem Cells. View Larger

Integrin alpha V/CD51 in Human Mesenchymal Stem Cells. Integrin aV/CD51 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human Integrin aV/CD51 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V/CD51

Integrin alpha V, also known as CD51 and vitronectin receptor subunit  alpha, is a 140 - 150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin  alpha V shares 92% aa sequence identity with mouse Integrin  alpha V ECD.

Entrez Gene IDs
3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142

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