Human Integrin alpha V/CD51 Biotinylated Antibody

BAF1219 Datasheet / COA / SDS

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BAF1219

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Human Integrin alpha V/CD51 Biotinylated Antibody in Data

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Human Integrin alpha V/CD51 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha V/CD51 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Integrin alpha V is observed, 5% cross‑reactivity with recombinant human (rh) Integrin alpha 2 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5 and rmIntegrin alpha E is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51
Phe31-Val992
Accession # NP_002201

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Integrin alpha V/CD51

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry

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Integrin alpha V/CD51 in Human Mesenchymal Stem Cells. Integrin aV/CD51 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human Integrin aV/CD51 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V/CD51

Integrin alpha V, also known as CD51 and vitronectin receptor subunit alpha, is a 140 - 150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin alpha V shares 92% aa sequence identity with mouse Integrin alpha V ECD.

Entrez Gene IDs

3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)

Alternate Names

antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142