Detects human Integrin alpha V/CD51 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Integrin alpha V is observed, 5% cross‑reactivity with recombinant human (rh) Integrin alpha 2 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5 and rmIntegrin alpha E is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51 Phe31-Val992 Accession # NP_002201
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Human Integrin alpha V/CD51
2.5 µg/106 cells
Human peripheral blood mononuclear cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Integrin alpha V/CD51 in Human Mesenchymal Stem Cells.
Integrin alpha V/CD51 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human Integrin alpha V/CD51 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha V/CD51
Integrin alpha V, also known as CD51 and vitronectin receptor subunit alpha, is
a 140 - 150 kDa integrin alpha chain that forms dimers with at least
five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid
(aa) residue type I membrane protein with a large (962 aa)
extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The
N-terminal region of alpha V, which is important for ligand binding,
contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl)
concensus repeats that fold into a beta -propellar domain. Furin cleavage
of the alpha V ECD occurs after Gly 889, generating a disulfide-linked,
heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM
molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD
of human Integrin alpha V shares 92% aa sequence identity with mouse
Integrin alpha V ECD.
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