Human/Mouse/Rat Integrin  alpha V/CD51 Antibody

Catalog # Availability Size / Price Qty
AF1219
AF1219-SP
Human/Mouse/Rat Integrin  alpha V/CD51 Antibody in Data
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Citations (3)
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Human/Mouse/Rat Integrin  alpha V/CD51 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Integrin  alpha V in direct ELISAs and Western blots. Detects human, mouse and rat Integrin  alpha V in Simple Western application. In Western blots, less than 1% cross-reactivity with recombinant human Integrin  alpha 5 and recombinant mouse Integrin E is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin  alpha V/CD51
Phe31-Val992
Accession # P06756
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
20 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Knockout Validated
Integrin  alpha V/CD51 is specifically detected in A549 human lung carcinoma parental cell line but is not detectable in Integrin  alpha V/CD51 knockout A549 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Integrin aV/CD51 antibody by Western Blot. View Larger

Detection of Human Integrin alpha V/CD51 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin aV/CD51 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Integrin aV/CD51 antibody in Rat Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Integrin alpha V/CD51 in Rat Mesenchymal Stem Cells. Integrin aV/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Human, Mouse, and Rat Integrin aV/CD51 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human, Mouse, and Rat Integrin alpha V/CD51 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin aV/CD51 at approximately 149-161 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human Integrin aV/CD51 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Integrin alpha V/CD51 Specificity by Using Knockout Cell Line. Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin aV/CD51 knockout A549 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Integrin aV/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin aV/CD51 at approximately 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V/CD51

Integrin alpha V, also known as CD51 and vitronectin receptor subunit  alpha, is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin  alpha V shares 92% aa sequence identity with mouse Integrin  alpha V ECD.

Entrez Gene IDs
3685 (Human); 16410 (Mouse); 296456 (Rat); 102116249 (Cynomolgus Monkey)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITGAV; MSK8; Vitronectin Receptor alpha; Vitronectin receptor subunit alpha; VNRA; VNRADKFZp686A08142

Product Datasheets

Citations for Human/Mouse/Rat Integrin  alpha V/CD51 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Characterization of human and murine T-cell immunoglobulin mucin domain 4 (TIM-4) IgV domain residues critical for Ebola virus entry
    J Virol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Quantity and accessibility for specific targeting of receptors in tumours.
    Authors: Hussain, Sajid, Rodriguez-Fernandez, Maria, Braun, Gary B, Doyle, Francis, Ruoslahti, Erkki
    Sci Rep, 2014;4(0):5232.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  3. Binding of extracellular maspin to beta1 integrins inhibits vascular smooth muscle cell migration.
    Authors: Bass R, Wagstaff L, Ravenhill L, Ellis V
    J. Biol. Chem., 2009;284(40):27712-20.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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