Detects human Integrin alpha V in direct ELISAs and Western blots. Detects human, mouse and rat Integrin alpha V in Simple Western application. In Western blots, less than 1% cross-reactivity with recombinant human Integrin alpha 5 and recombinant mouse Integrin E is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha V/CD51 Phe31-Val992 Accession # P06756
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Human peripheral blood mononuclear cells
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Integrin alpha V/CD51 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin alpha V/CD51 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Integrin alpha V/CD51 in Rat Mesenchymal Stem Cells. Integrin alpha V/CD51 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Human/Mouse/Rat Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human, Mouse, and Rat Integrin alpha V/CD51 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, A549 human lung carcinoma cell line, C6 rat glioma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha V/CD51 at approximately 149-161 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Integrin alpha V/CD51 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1219) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha V/CD51
Integrin alpha V, also known as CD51 and vitronectin receptor subunit alpha, is a 140-150 kDa integrin alpha chain that forms dimers with at least five beta chains including beta 1, 3, 5, 6, and 8. It is a 1018 amino acid (aa) residue type I membrane protein with a large (962 aa) extracellular domain (ECD) and a short (32 aa) cytoplasmic tail. The N-terminal region of alpha V, which is important for ligand binding, contains seven FG-GAP (phenylalanyl-glycyl and glycyl-alanyl-prolyl) concensus repeats that fold into a beta -propellar domain. Furin cleavage of the alpha V ECD occurs after Gly 889, generating a disulfide-linked, heteromeric subunit alpha V chain. alpha V-containing integrins bind multiple ECM molecules, including vitronectin, osteopontin, MMP-2, and TSP. The ECD of human Integrin alpha V shares 92% aa sequence identity with mouse Integrin alpha V ECD.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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