Human Integrin  beta 2/CD18 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1730P
Human Integrin  beta 2/CD18 PE-conjugated Antibody in Data
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Citations (3)
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Human Integrin  beta 2/CD18 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 2/CD18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin  beta 1 or recombinant mouse Integrin  alpha 5 is observed.
Source
Monoclonal Mouse IgG1 Clone # 212701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin  beta 2/CD18
Gln23-Asn700
Accession # AAA59490
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Integrin  beta 2/CD18 antibody in Human Peripheral Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of Integrin beta 2/CD18 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood Lymphocytes were stained with Mouse Anti-Human Integrin beta 2/CD18 PE-conjugated Monoclonal Antibody (Catalog # FAB1730P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin beta 2/CD18

Integrin alpha X beta 2, also called CD11c/CD18, p150/95 or complement receptor type 4 (CR4), is one of four beta 2 integrins. The non-covalent heterodimer of 150 kDa alpha X/CD11c and 95 kDa beta 2/CD18 integrin subunits is expressed on macrophages, dendritic cells and hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells (1‑7). Like other integrins, alpha X beta 2 has multiple activation states (3). In the presence of divalent cations and "inside-out" signaling, alpha X beta 2 is fully active and extended. The alpha X vWFA or I-domain, which contains the adhesion sites, forms the N-terminal head region with the alpha X beta-propeller and the beta 2 vWFA domain (1, 8). In the inactive state, the heterodimer flexes in the center at the alpha X thigh and calf domains and beta 2 I-EGF domains, impeding access to adhesion sites (1). The 1088 aa human alpha X/CD11c ECD shares 70‑76% aa sequence identity with mouse, rat and canine alpha X while the 678 aa human beta 2/CD18 ECD shares 81‑83% aa sequence identity with mouse, rat, cow, dog, goat, sheep, and pig beta 2. Potential alpha X isoforms containing 719 and 725 aa (as compared to full-length 1163 aa alpha X) lack the vWFA domain and the N-terminus. Active alpha X beta 2 shares some adhesion partners with alpha M beta 2/CD11b/CD18, including complement opsonin fragment iC3b, ICAMs, vWF and fibrinogen, and is expressed on many of the same cells (4‑11). However, alpha M beta 2 activity is often constitutive, while alpha X beta 2 activity requires cell activation (4‑7). alpha X beta 2 also binds osteopontin, Thy-1, plasminogen, heparin, and proteins with abnormally exposed acidic residues (11‑16). The adhesion events are important for proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles (5, 6, 9, 11, 12, 16). Mutations of beta 2, especially in the vWFA domain, cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections (17).

References
  1. Corbi, A.L. et al. (1987) EMBO J. 6:4023.
  2. Kishimoto, T.K. et al. (1987) Cell 48:681.
  3. Hynes, R.O. (2002) Cell 110:673.
  4. Arnaout, M.A. (1990) Blood 75:1037.
  5. Postigo, A.A. et al. (1991) J. Exp. Med. 174:1313.
  6. Beyer, M. et al. (2005) Respir. Res. 6:70.
  7. Nicolaou, F. et al. (2003) Blood 101:4033.
  8. Vorup-Jensen, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1873.
  9. Bilsland, C.A.G. et al. (1994) J. Immunol. 152:4582.
  10. Pendu, R. et al. (2006) Blood 108:3746.
  11. Sadhu, C. et al. (2007) J. Leukoc. Biol. 81:1395.
  12. Schack, L. et al. (2009) J. Immunol. 182:6943.
  13. Choi, J. et al. (2005) Biochem. Biophys. Res. Commun. 331:557.
  14. Gang, J. et al. (2007) Mol. Cells 24:240.
  15. Vorup-Jensen, T. et al. (2007) J. Biol. Chem. 282:30869.
  16. Vorup-Jensen, T. et al. (2004) Proc. Natl. Acad. Sci. USA 102:1614.
  17. Kishimoto, T.K. et al. (1987) Cell 50:193.
Entrez Gene IDs
3689 (Human); 16414 (Mouse); 309684 (Rat); 101925124 (Cynomolgus Monkey)
Alternate Names
959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta

Product Datasheets

Citations for Human Integrin  beta 2/CD18 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Origin-Specific Adhesive Interactions of Mesenchymal Stem Cells with Platelets Influence Their Behavior After Infusion
    Authors: L Sheriff, A Alanazi, LSC Ward, C Ward, H Munir, J Rayes, M Alassiri, SP Watson, PN Newsome, GE Rainger, N Kalia, J Frampton, HM McGettrick, GB Nash
    Stem Cells, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro.
    Authors: Ghislin S, Obino D, Middendorp S, Boggetto N, Alcaide-Loridan C, Deshayes F
    BMC Cancer, 2012;12(0):455.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011;9(5):1056-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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