Human KIR3DL1 APC-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human KIR3DL1. Did not cross-react with other KIR family members when tested against a series of KIR-expressing BaF/3 tansfectants (KIR2DL1, KIR2DL2, KIR2DL3, KIR2DL4, KIR2DS1, KIR2DS2, KIR2DS3, KIR2DS4, and KIR3DL2; Bakker, A.B.H. et al. (1998) J. Immunol. 160:5239).
  • Source
    Monoclonal Mouse IgG1 Clone # DX9
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human KIR3DL1-expressing NK cell clone
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of KIR3DL1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR3DL1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1225A) and Mouse Anti-Human
NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: KIR3DL1

KIR3DL1 (3DL1, previously called NKB1 or NKAT3, designated CD158e) is a 70 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1‑3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Like other inhibiting KIRs, KIR3DL1 has two ITIM domains within its long tail (2). The 319 amino acid (aa) ECD of KIR3DL1 shows 97% aa identity with an activating KIR, KIR3DS1, and the two segregate as alleles (3, 4). KIR3DL1 binds to HLA antigens. This includes HLA-A and -B molecules. Among the HLA-B variants, only the Bw4 epitope, which is present within only one third of all HLA-B alleles, is recognized by KIR3DL1 (4). An NK cell expressing KIR3DL1 is prevented from killing a cell expressing the Bw4 epitope on its surface. However, if the epitope is downregulated on the cell surface due to viral infection, the NK cell is released from inhibition and now kills the infected cell. KIR genes are highly polymorphic, and specific KIR3DL1 alleles vary in surface expression and activity. For example, the allele KIR3DL1*004 is associated with slow progression to AIDS in HIV infected individuals that also express Bw4 (6). Unlike most alleles that are surface-expressed, this allele is mainly retained within the cell (7). KIR3DL1/S1 is the only KIR receptor to have an ortholog in non-primates, including selected mouse strains in which it is also called KIRL1 (KIR-like 1). Although the ECD of human KIR3DL1 shares 40‑48% aa identity with mouse, rat and bovine KIR3DL1, the transmembrane and cytoplasmic regions in the non-primate species show no obvious activating or inhibiting motifs (8, 9).

  • References:
    1. Colonna, M. and J. Samaridis (1995) Science 268:405.
    2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
    3. Uhrberg, M. et al. (1997) Immunity 7:753.
    4. O’Connor, G. M. et al. (2007) J. Immunol. 178:235.
    5. Thananchai, H. et al. (2007) J. Immunol. 178:33.
    6. Martin, M.P. et al. (2007) Nat. Genet. 39:733.
    7. Pando, M.J. et al. (2003) J. Immunol. 171:6640.
    8. Hoelsbrekken, S.E. et al. (2003) J. Immunol. 170:2259.
    9. Wilson, E.B. et al. (2007) Immunogenetics 59:641.
  • Long Name:
    Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 1
  • Entrez Gene IDs:
    3811 (Human)
  • Alternate Names:
    AMB11; CD158 antigen-like family member E; CD158e antigen; CD158E; CD158e1; CD158e1/2; CD158e2; cl-11; cl-2; HLA-BW4-specific inhibitory NK cell receptor; killer cell immunoglobulin-like receptor 3DL1; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail1,nkat3; killer Ig receptor; KIR antigen 3DL1; KIR3DL1/S1; KIR3DS1; MGC119726; MGC119728; MGC126589; MGC126591; MHC class I NK cell receptor; Natural killer-associated transcript 3; Nkat3; NKAT-3; NKAT3KIR; NKB1; NKB1B; NKB1CD158E1; NK-receptor; p70 killer cell inhibitory receptor; p70 natural killer cell receptor clones CL-2/CL-11; p70 NK receptor CL-2/CL-11
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. The Incubation Period of Primary Epstein-Barr Virus Infection: Viral Dynamics and Immunologic Events.
    Authors: Dunmire S, Grimm J, Schmeling D, Balfour H, Hogquist K
    PLoS Pathog, 2015;11(12):e1005286.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Expansion of CMV-mediated NKG2C+ NK cells associates with the development of specific de novo malignancies in liver-transplanted patients.
    Authors: Achour, Abla, Baychelier, Florence, Besson, Caroline, Arnoux, Armelle, Marty, Michel, Hannoun, Laurent, Samuel, Didier, Debre, Patrice, Vieillard, Vincent
    J Immunol, 2014;192(1):503-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010;5(8):e11966.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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