Human KIR3DL1 Antibody

Catalog # Availability Size / Price Qty
MAB1225
MAB1225-SP
Product Details
Citations (2)
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Human KIR3DL1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR3DL1. Did not cross-react with other KIR family members when tested against a series of KIR-expressing BaF/3 tansfectants (KIR2DL1, KIR2DL2, KIR2DL3, KIR2DL4, KIR2DS1, KIR2DS2, KIR2DS3, KIR2DS4, and KIR3DL2; Bakker, A.B.H. et al. (1998) J. Immunol. 160:5239).
Source
Monoclonal Mouse IgG1 Clone # DX9
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human KIR3DL1-expressing NK cell clone
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Human whole blood CD56+ natural killer cells
Blockade of Receptor-ligand Interaction
This antibody has been used to block the interaction of NK cell-surface KIR3DL1 with selected HLA-B alleles, thereby preventing KIR3DL1 inhibitory activity (Litwin, V. et al. (1994) J. Exp. Med. 180:537).
 
CyTOF-reported
Horowitz, A. et al. (2015) J. Immunol. 195: 4524. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR3DL1

KIR3DL1 (3DL1, previously called NKB1 or NKAT3, designated CD158e) is a 70 kDa type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they regulate effector functions in the innate immune system (1‑3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. Like other inhibiting KIRs, KIR3DL1 has two ITIM domains within its long tail (2). The 319 amino acid (aa) ECD of KIR3DL1 shows 97% aa identity with an activating KIR, KIR3DS1, and the two segregate as alleles (3, 4). KIR3DL1 binds to HLA antigens. This includes HLA-A and -B molecules. Among the HLA-B variants, only the Bw4 epitope, which is present within only one third of all HLA-B alleles, is recognized by KIR3DL1 (4). An NK cell expressing KIR3DL1 is prevented from killing a cell expressing the Bw4 epitope on its surface. However, if the epitope is downregulated on the cell surface due to viral infection, the NK cell is released from inhibition and now kills the infected cell. KIR genes are highly polymorphic, and specific KIR3DL1 alleles vary in surface expression and activity. For example, the allele KIR3DL1*004 is associated with slow progression to AIDS in HIV infected individuals that also express Bw4 (6). Unlike most alleles that are surface-expressed, this allele is mainly retained within the cell (7). KIR3DL1/S1 is the only KIR receptor to have an ortholog in non-primates, including selected mouse strains in which it is also called KIRL1 (KIR-like 1). Although the ECD of human KIR3DL1 shares 40‑48% aa identity with mouse, rat and bovine KIR3DL1, the transmembrane and cytoplasmic regions in the non-primate species show no obvious activating or inhibiting motifs (8, 9).

References
  1. Colonna, M. and J. Samaridis (1995) Science 268:405.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Uhrberg, M. et al. (1997) Immunity 7:753.
  4. O’Connor, G. M. et al. (2007) J. Immunol. 178:235.
  5. Thananchai, H. et al. (2007) J. Immunol. 178:33.
  6. Martin, M.P. et al. (2007) Nat. Genet. 39:733.
  7. Pando, M.J. et al. (2003) J. Immunol. 171:6640.
  8. Hoelsbrekken, S.E. et al. (2003) J. Immunol. 170:2259.
  9. Wilson, E.B. et al. (2007) Immunogenetics 59:641.
Long Name
Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 1
Entrez Gene IDs
3811 (Human)
Alternate Names
AMB11; CD158 antigen-like family member E; CD158e antigen; CD158E; CD158e1; CD158e1/2; CD158e2; cl-11; cl-2; HLA-BW4-specific inhibitory NK cell receptor; killer cell immunoglobulin-like receptor 3DL1; killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail1,nkat3; killer Ig receptor; KIR antigen 3DL1; KIR3DL1; KIR3DL1/S1; KIR3DS1; MGC119726; MGC119728; MGC126589; MGC126591; MHC class I NK cell receptor; Natural killer-associated transcript 3; Nkat3; NKAT-3; NKAT3KIR; NKB1; NKB1B; NKB1CD158E1; NK-receptor; p70 killer cell inhibitory receptor; p70 natural killer cell receptor clones CL-2/CL-11; p70 NK receptor CL-2/CL-11

Product Datasheets

Citations for Human KIR3DL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets.
    Authors: Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA
    Blood, 2010;115(2):274-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.
    Authors: Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A
    Blood, 2007;111(3):1428-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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