Human KLF2 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross‑reactivity with rhKLF1, 4, 5, 6, 10, 12, recombinant mouse KLF4 or 15 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 665333
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human KLF2
    Pro71-Pro168 (predicted)
    Accession # Q9Y5W3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry

Detection of KLF2 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 APC‑conjugated Monoclonal Antibody (Catalog # IC5466A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: KLF2

KLF2 (Kruppel-Like Factor 2), also known as LKLF, is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family.  KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells.  It is a transcription factor that regulates multiple genes, many of which are involved in cell migration. Human KLF2 is 355 amino acids (aa) in length.  It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354).  There is one potential splice form that shows a premature truncation after Asp224. Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.  

  • Long Name:
    Kruppel-Like Factor 2
  • Entrez Gene IDs:
    10365 (Human); 16598 (Mouse); 306330 (Rat)
  • Alternate Names:
    Krueppel-like factor 2; Kruppel-like factor 2 (lung); Kruppel-like factor LKLF; LKLF; LKLFLung krueppel-like factor; lung Kruppel-like zinc finger transcription factor
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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