Human KLF2 Antibody

Detection of Human KLF2 by Immunocytochemistry/ Immunofluorescence

Characterization of CCM1+/− and clonally expanded CCM1−/− BOECS. (A)CCM1−/− BOECs were established by limiting dilution of the CRISPR/Cas9 RNP-treated cell pool and clonal expansion of single cells (created with BioRender.com). (B) Shown are the genotypes of CCM1−/− BOEC clones included in this study. All variants either lead to a frameshift or a premature stop codon (additional information on the CRISPR/Cas9-induced mutations and numbers of individual clones are given in Supplementary Table S1). (C)CCM1−/− BOECs presented a more compact morphology in brightfield microscopy and increased tube formation. The largest cell diameter and the number of meshes formed on Matrigel-coated plates were quantified. Scale bar indicates 400 µm. Data are presented as single data points with the mean (n = 4–6). (D) Immunofluorescence staining revealed a higher number of intercellular gaps (white arrowheads), more actin stress fibers, and a higher expression of KLF2 and KLF4 in CCM1−/− BOECs. Representative images are shown. Scale bar indicates 100 µm. Individual data points are shown with the mean (n = 3–5). Student’s t test was used for statistical analysis: *p < 0.05; **p < 0.01; ****p < 0.0001; ns = not significant; VE-cadherin = vascular endothelial cadherin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34307446), licensed under a CC-BY license. Not internally tested by R&D Systems.