Intracellular Staining by Flow Cytometry
|Detection of KLF2 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 PE‑conjugated Monoclonal Antibody (Catalog # IC5466P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
KLF2 (Kruppel-Like Factor 2), also known as LKLF, is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family. KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells. It is a transcription factor that regulates multiple genes, many of which are involved in cell migration. Human KLF2 is 355 amino acids (aa) in length. It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354). There is one potential splice form that shows a premature truncation after Asp224. Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.
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