Human KLF2 PE-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross‑reactivity with rhKLF1, 4, 5, 6, 10, 12, recombinant mouse KLF4 or 15 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 665333
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human KLF2
    Pro71-Pro168 (predicted)
    Accession # Q9Y5W3
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of KLF2 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 PE‑conjugated Monoclonal Antibody (Catalog # IC5466P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: KLF2

KLF2 (Kruppel-Like Factor 2), also known as LKLF, is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family.  KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells.  It is a transcription factor that regulates multiple genes, many of which are involved in cell migration. Human KLF2 is 355 amino acids (aa) in length.  It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354).  There is one potential splice form that shows a premature truncation after Asp224. Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.  

  • Long Name:
    Kruppel-Like Factor 2
  • Entrez Gene IDs:
    10365 (Human); 16598 (Mouse); 306330 (Rat)
  • Alternate Names:
    KLF2; Krueppel-like factor 2; Kruppel-like factor 2 (lung); Kruppel-like factor LKLF; LKLF; LKLFLung krueppel-like factor; lung Kruppel-like zinc finger transcription factor
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. High pathogen burden in childhood promotes the development of unconventional innate-like CD8+ T cells
    Authors: YT Falanga, M Frascoli, Y Kaymaz, C Forconi, JM Ong'echa, JA Bailey, LJ Berg, AM Moormann
    JCI Insight, 2017;2(15):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 20  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human KLF2 PE-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information


Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

R&D Systems Guarantee

Panels For

Learn More
Monoclonal Antibodies


Customer Information