Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 665333
View all KLF2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KLF2
Pro71-Pro168 (predicted)
Accession # Q9Y5W3

Specificity

Detects human KLF2 in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) KLF17 and no cross‑reactivity with rhKLF1, 4, 5, 6, 10, 12, recombinant mouse KLF4 or 15 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human KLF2 PE‑conjugated Antibody

Detection of KLF2 antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of KLF2 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Mouse Anti-Human KLF2 PE-conjugated Monoclonal Antibody (Catalog # IC5466P, filled histogram) or isotype control antibody (IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of KLF2 in human iPSCs by Flow Cytometry

human iPSCs were stained with Mouse Anti-Human KLF2 PE‑conjugated Monoclonal Antibody (Catalog # IC5466P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human KLF2 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: BG01V human embryonic stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KLF2

KLF2 (Kruppel-Like Factor 2), also known as LKLF, is a lung-associated, 37 kDa member of the Kruppel C2H2-type zinc-finger protein family.  KLF2 is found in airway epithelium, endothelium, monocytes, T and B cells.  It is a transcription factor that regulates multiple genes, many of which are involved in cell migration. Human KLF2 is 355 amino acids (aa) in length.  It contains an activation domain (aa 1-110), an inhibitory domain (aa 111-267), and three C2H2-type zinc-finger regions (aa 272-354).  There is one potential splice form that shows a premature truncation after Asp224. Over aa 71-168, human KLF2 is 82% aa identical to mouse KLF2.  

Long Name

Kruppel-Like Factor 2

Alternate Names

LKLF

Entrez Gene IDs

10365 (Human); 16598 (Mouse); 306330 (Rat)

Gene Symbol

KLF2

UniProt

Q9Y5W3 (Human)

Additional KLF2 Products

Product Documents for Human KLF2 PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human KLF2 PE‑conjugated Antibody

For research use only

Citations for Human KLF2 PE‑conjugated Antibody

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