Human KLF4 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC3640A
Human KLF4 APC-conjugated Antibody in Intracellular Staining by Flow Cytometry
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Product Details
Citations (1)
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Human KLF4 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) KLF4 and less than 1% cross-reactivity with recombinant human (rh) KLF5, rhKLF6, and rmKLF15.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human KLF4
Ala2-Phe470
Accession # AAH29923
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of KLF4 in BG01V Human Cells by Flow Cytometry. View Larger

Detection of KLF4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Goat Anti-Human KLF4 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3640A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KLF4

Human KLF4, also known as Epithelial Zinc Finger protein (EZF), is a 53 kDa (470 aa) member of the kruppel C2H2-type zinc finger protein family. It contains three C2H2-type zinc fingers at the carboxyl terminus that preferentially bind to cis-DNA elements that are GC rich. KLF4 regulates the expression of target genes that are involved in different cellular functions. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract. Human and mouse KLF4 share 90% amino acid sequence identity.

Long Name
Kruppel-Like Factor 4
Entrez Gene IDs
9314 (Human)
Alternate Names
Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut)

Product Datasheets

Citation for Human KLF4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Molecular iodine inhibits the expression of stemness markers on cancer stem-like cells of established cell lines derived from cervical cancer
    Authors: GD Bigoni-Ord, E Ortiz-Sánc, P Rosendo-Ch, HA Valencia-G, C Aceves, A García-Car
    BMC Cancer, 2018;18(1):928.
    Species: Human
    Sample Types: Whole Cells

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