Human KLF4 Antibody

Catalog # Availability Size / Price Qty
AF3640
AF3640-SP
Human KLF4 Antibody in Western Blot
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Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (1)

Human KLF4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KLF4 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 30% cross‑reactivity with recombinant mouse KLF4.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human KLF4
Ala2-Phe470
Accession # AAH29923
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
25 µg/mL
See below
Immunohistochemistry
15 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human KLF4 by Western Blot. View Larger

Detection of Human KLF4 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, SW480 human colorectal adenocarcinoma cell line, and HCT-116 human colorectal carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for KLF4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Immunocytochemistry KLF4 in BG01V Human Stem Cells. View Larger

KLF4 in BG01V Human Stem Cells. KLF4 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Chromatin Immunoprecipitation (ChIP) Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. View Larger

Detection of KLF4-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. TheB2Rpromoter was detected by standard PCR.

Immunohistochemistry KLF4 in Human Colon. View Larger

KLF4 in Human Colon. KLF4 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human KLF4 by Simple Western<sup>TM</sup>. View Larger

Detection of Human KLF4 by Simple WesternTM. Simple Western lane view shows lysates of HT-29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for KLF4 at approximately 63 kDa (as indicated) using 25 µg/mL of Goat Anti-Human KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3640) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KLF4

Human KLF4, also known as epithelial zinc finger protein (EZF), is a 53 kDa (470 aa) member of the kruppel C2H2-type zinc finger protein family. It contains three C2H2-type zinc fingers at the carboxyl terminus that preferentially bind to cis-DNA elements that are GC rich. KLF4 regulates the expression of target genes that are involved in different cellular functions. KLF4 is highly expressed in the epithelial cells of the skin and the gastrointestinal tract. Human and mouse KLF4 share 90% amino acid sequence identity.

Long Name
Kruppel-Like Factor 4
Entrez Gene IDs
9314 (Human)
Alternate Names
Epithelial zinc finger protein EZF; EZF; EZFendothelial Kruppel-like zinc finger protein; GKLFKrueppel-like factor 4; Gut-enriched krueppel-like factor; KLF4; Kruppel-like factor 4 (gut)

Product Datasheets

Citations for Human KLF4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Measles vector as a multiple genes delivery platform facilitating iPSC reprogramming
    Authors: Q Wang, A Vossen, Y Ikeda, P Devaux
    Gene Ther., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs
    Authors: J Pontis, E Planet, S Offner, P Turelli, J Duc, A Coudray, TW Theunissen, R Jaenisch, D Trono
    Cell Stem Cell, 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  3. p63 establishes epithelial enhancers at critical craniofacial development genes
    Authors: E Lin-Shiao, Y Lan, J Welzenbach, KA Alexander, Z Zhang, M Knapp, E Mangold, M Sammons, KU Ludwig, SL Berger
    Sci Adv, 2019;5(5):eaaw0946.
    Species: Human
    Sample Types:
    Applications: ChIP
  4. AAVvector-mediated in vivo reprogramming into pluripotency
    Authors: E Senís, L Mosteiro, S Wilkening, E Wiedtke, A Nowrouzi, S Afzal, R Fronza, H Landerer, M Abad, D Niopek, M Schmidt, M Serrano, D Grimm
    Nat Commun, 2018;9(1):2651.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Data in support of sustained upregulation of adaptive redox homeostasis mechanisms caused by KRIT1 loss-of-function
    Authors: C Antognelli, E Trapani, S Delle Mona, A Perrelli, C Fornelli, F Retta, P Cassoni, VN Talesa, SF Retta
    Data Brief, 2018;16(0):929-938.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The TFAP2C-Regulated OCT4 Naive Enhancer Is Involved in Human Germline Formation
    Authors: D Chen, W Liu, J Zimmerman, WA Pastor, R Kim, L Hosohama, J Ho, M Aslanyan, JJ Gell, SE Jacobsen, AT Clark
    Cell Rep, 2018;25(13):3591-3602.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Kruppel-like factor 4 regulates keratinocyte senescence
    Authors: E Panatta, AM Lena, M Mancini, M Affinati, A Smirnov, M Annicchiar, MC Piro, E Campione, L Bianchi, C Mazzanti, G Melino, E Candi
    Biochem. Biophys. Res. Commun., 2018;0(0):.
    Species: Human
    Sample Types: keratinocytes
    Applications: Western Blot
  8. KLF2 and KLF4 control endothelial identity and vascular integrity
    Authors: P Sangwung, G Zhou, L Nayak, ER Chan, S Kumar, DW Kang, R Zhang, X Liao, Y Lu, K Sugi, H Fujioka, H Shi, SD Lapping, CC Ghosh, SJ Higgins, SM Parikh, H Jo, MK Jain
    JCI Insight, 2017;2(4):e91700.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer
    Authors: P Wang, WW Wan, SL Xiong, H Feng, N Wu
    Cell Death Discov, 2017;3(0):16105.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability.
    Authors: Liu L, Wu L, Wei X, Ling J
    Stem Cells Dev, 2015;24(8):962-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  12. Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
    Authors: Park D, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong K, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong Y, Koh G
    J Clin Invest, 2014;124(9):3960-74.
  13. ZNF750 interacts with KLF4 and RCOR1, KDM1A, and CTBP1/2 chromatin regulators to repress epidermal progenitor genes and induce differentiation genes.
    Authors: Boxer L, Barajas B, Tao S, Zhang J, Khavari P
    Genes Dev, 2014;28(18):2013-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  14. KLF4 modulates expression of IL-6 in dendritic cells via both promoter activation and epigenetic modification.
    Authors: Rosenzweig, Jason M, Glenn, Justin D, Calabresi, Peter A, Whartenby, Katharin
    J Biol Chem, 2013;288(33):23868-74.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Mobility Shift Assay
  15. Endogenous Purification Reveals GREB1 as a Key Estrogen Receptor Regulatory Factor.
    Authors: Mohammed H
    Oncogene, 2012;3(2):342-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  16. Innate immune suppression enables frequent transfection with RNA encoding reprogramming proteins.
    Authors: Angel M, Yanik MF,
    PLoS ONE, 2010;5(7):e11756.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human KLF4 Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: A172 human glioblastoma cell line Species: Human