Human LAIR1 Alexa Fluor® 488-conjugated Antibody

FAB2664G Datasheet / COA / SDS

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FAB2664G-100UG

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Human LAIR1 Alexa Fluor® 488-conjugated Antibody in Data

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Human LAIR1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAIR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAIR2 or recombinant mouse LAIR1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 342219

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAIR1 isoform 1
Gln22-His163
Accession # Q6GTX8

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of LAIR1 in Human Lymphocytes by Flow Cytometry. Human lymphocytes were stained with Mouse Anti-Human LAIR1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2664G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitution default not known

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1; also CD305) is a 46 kDa inhibitory receptor belonging to the Ig superfamily. It is a type I transmembrane protein with one extracellular Ig-like domain and two cytoplasmic ITIMs. Four LAIR-1 splice variants exist. LAIR1b has a 17 aa deletion outside the Ig loop in the extracellular domain. It differs from LAIR1c by one aa residue. LAIR1d has a 77 aa truncation in the cytoplasmic domain. LAIR1 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells and most thymocytes. The extracellular domain of human LAIR1 shares 40% aa identity with that of the mouse protein.

Long Name

Leukocyte-associated Ig-like Receptor 1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Alternate Names

CD305 antigen; CD305; CD305leukocyte-associated Ig-like receptor 1; HLAIR1; LAIR1; LAIR-1; leukocyte-associated immunoglobulin-like receptor 1