Human LAP TGF-beta 1 Biotinylated Antibody

  (8 citations)     
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Citations (8)
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  • Species Reactivity
    Human
  • Specificity
    Detects human LAP TGF-beta 1 in Western blots. In this format, less than 1% cross-reactivity with mature recombinant human (rh) TGF‑ beta 1, porcine TGF‑ beta 2, rhTGF‑ beta 3, and recombinant amphibian TGF‑ beta 5 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP TGF‑ beta 1 and Chinese hamster ovary cell line CHO-derived recombinant human LAP TGF‑ beta 1
    Leu30-Ser390
    Accession # P01137
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAP (TGF-beta 1)

TGF-beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta  superfamily. TGF-  beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N‑terminal 249 aa (aa 30-278) latency-associated peptide (LAP) and a C-terminal 112 aa (aa 279-390) mature TGF-  beta 1 (4-6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non‑covalently associated after secretion, forming the small latent TGF-beta 1 complex (4-8). Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5‑7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1 (10).

  • References:
    1. Dunker, N. & K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
    2. Wahl, S.M. (2006) Immunol. Rev. 213:213.
    3. Chang, H. et al. (2002) Endocr. Rev. 23:787.
    4. Derynck, R. et al. (1985) Nature 316:701.
    5. Dabovic, B. and D.B. Rifkin (2008) “TGF-beta Bioavailability” in The TGF-beta Family. Derynck, R. and K. Miyazono (eds): Cold Spring Harbor Laboratory Press, p. 179.
    6. Brunner, A.M. et al. (1989) J. Biol. Chem. 264:13660.
    7. Miyazono, K. et al. (1991) EMBO J. 10:1091.
    8. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
    9. Miller, D.M. et al. (1992) Mol. Endocrinol. 6:694.
    10. Janssens, K. et al. (2003) J. Biol. Chem. 278:7718.
  • Long Name:
    Latency-associated Peptide
  • Entrez Gene IDs:
    7040 (Human)
  • Alternate Names:
    CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor beta1 (TGF-beta1) Production in Human Regulatory T Cells.
    Authors: Huygens C, Lienart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, Lucas S
    J Biol Chem, 2015;290(33):20105-16.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
    Authors: Oida T, Weiner HL
    J. Immunol., 2012;185(6):3529-35.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Involvement of regulatory T cells in the immunosuppression characteristic of patients with paracoccidioidomycosis.
    Authors: Ferreira MC, de Oliveira RT, da Silva RM, Blotta MH, Mamoni RL
    Infect. Immun., 2010;78(10):4392-401.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens.
    Authors: Burton OT, Gibbs S, Miller N, Jones FM, Wen L, Dunne DW, Cooke A, Zaccone P
    Eur. J. Immunol., 2010;40(8):2221-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Membrane protein GARP is a receptor for latent TGF-beta on the surface of activated human Treg.
    Authors: Stockis J, Colau D, Coulie PG, Lucas S
    Eur. J. Immunol., 2009;39(12):3315-22.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. T-cell-expressed proprotein convertase furin is essential for maintenance of peripheral immune tolerance.
    Authors: Pesu M, Watford WT, Wei L, Xu L, Fuss I, Strober W, Andersson J, Shevach EM, Quezado M, Bouladoux N, Roebroek A, Belkaid Y, Creemers J, O'Shea JJ
    Nature, 2008;455(7210):246-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
    Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
    J. Immunol., 2004;172(9):5753-64.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Tissue Homogenates
    Application: WB
  8. Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA.
    Authors: La Cava A, Ebling FM, Hahn BH
    J. Immunol., 2004;173(5):3542-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

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