Human LAP TGF-beta 1 Biotinylated Antibody

BAF246 8 Citations Datasheet / COA / SDS

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BAF246

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Citations (8)

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Human LAP TGF-beta 1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAP TGF-beta 1 in Western blots. In this format, less than 1% cross-reactivity with mature recombinant human (rh) TGF‑ beta 1, porcine TGF‑ beta 2, rhTGF‑ beta 3, and recombinant amphibian TGF‑ beta 5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP TGF‑ beta 1 and Chinese hamster ovary cell line CHO-derived recombinant human LAP TGF‑ beta 1
Leu30-Ser390
Accession # P01137

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAP (TGF-beta 1)

TGF-beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta superfamily. TGF- beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N‑terminal 249 aa (aa 30-278) latency-associated peptide (LAP) and a C-terminal 112 aa (aa 279-390) mature TGF- beta 1 (4-6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non‑covalently associated after secretion, forming the small latent TGF-beta 1 complex (4-8). Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5‑7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1 (10).

References

Dunker, N. & K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
Wahl, S.M. (2006) Immunol. Rev. 213:213.
Chang, H. et al. (2002) Endocr. Rev. 23:787.
Derynck, R. et al. (1985) Nature 316:701.
Dabovic, B. and D.B. Rifkin (2008) “TGF-beta Bioavailability” in The TGF-beta Family. Derynck, R. and K. Miyazono (eds): Cold Spring Harbor Laboratory Press, p. 179.
Brunner, A.M. et al. (1989) J. Biol. Chem. 264:13660.
Miyazono, K. et al. (1991) EMBO J. 10:1091.
Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
Miller, D.M. et al. (1992) Mol. Endocrinol. 6:694.
Janssens, K. et al. (2003) J. Biol. Chem. 278:7718.

Long Name

Latency-associated Peptide

Entrez Gene IDs

7040 (Human)

Alternate Names

CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1

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Related Research Areas

Citations for Human LAP TGF-beta 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Lysosomal-associated Transmembrane Protein 4B (LAPTM4B) Decreases Transforming Growth Factor beta1 (TGF-beta1) Production in Human Regulatory T Cells.
Authors: Huygens C, Lienart S, Dedobbeleer O, Stockis J, Gauthy E, Coulie P, Lucas S
J Biol Chem, 2015;290(33):20105-16.
Species: Human
Sample Types: Cell Lysates
Applications: WB
Overexpression of TGF-ÃŸ 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ÃŸ.
Authors: Oida T, Weiner HL
J. Immunol., 2012;185(6):3529-35.
Species: Human
Sample Types: Cell Lysates
Applications: WB
Importance of TLR2 in the direct response of T lymphocytes to Schistosoma mansoni antigens.
Authors: Burton OT, Gibbs S, Miller N, Jones FM, Wen L, Dunne DW, Cooke A, Zaccone P
Eur. J. Immunol., 2010;40(8):2221-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow
Involvement of regulatory T cells in the immunosuppression characteristic of patients with paracoccidioidomycosis.
Authors: Ferreira MC, de Oliveira RT, da Silva RM, Blotta MH, Mamoni RL
Infect. Immun., 2010;78(10):4392-401.
Species: Human
Sample Types: Whole Cells
Applications: Flow
Membrane protein GARP is a receptor for latent TGF-beta on the surface of activated human Treg.
Authors: Stockis J, Colau D, Coulie PG, Lucas S
Eur. J. Immunol., 2009;39(12):3315-22.
Species: Human
Sample Types: Cell Lysates
Applications: WB
T-cell-expressed proprotein convertase furin is essential for maintenance of peripheral immune tolerance.
Authors: Pesu M, Watford WT, Wei L, Xu L, Fuss I, Strober W, Andersson J, Shevach EM, Quezado M, Bouladoux N, Roebroek A, Belkaid Y, Creemers J, O'Shea JJ
Nature, 2008;455(7210):246-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow
Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
J. Immunol., 2004;172(9):5753-64.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Tissue Homogenates
Applications: WB
Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA.
Authors: La Cava A, Ebling FM, Hahn BH
J. Immunol., 2004;173(5):3542-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow