Detects endogenous human LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular domain (ECD). It does not cross-react with recombinant human PTPRM, PTPRK, DEP1, or PTPR gamma.
Monoclonal Rat IgG1 Clone # 384727
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human LAR Ala27-Glu1251 Accession # NP_569707
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human LAR by Western Blot. Western blot shows lysates of CHP‑100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human LAR Monoclonal Antibody (Catalog # MAB3004) followed by HRP-conjugated Anti‑Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LAR at approximately 139 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Leukocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phosphatase, Receptor-type F (PTPRF), is an integral membrane protein with anon-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium.
Leukocyte Antigen-Related Tyrosine Phosphatase
Entrez Gene IDs:
5792 (Human); 104121 (Mouse)
LCA-homolog; protein tyrosine phosphatase, receptor type, F; PTPRF; receptor type, F polypeptide; receptor-linked protein-tyrosine phosphatase LAR; receptor-type tyrosine-protein phosphatase F
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