Human LIF R alpha APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human LIF R alpha in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 32953
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LIF R alpha
    Accession # P42702
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LIF R alpha in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human LIF R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB249A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LIF R alpha

The activities of the pleiotropic cytokine LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha  subunit (LIF R alpha, also known as LIF R and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex. Besides LIF, the high-affinity heterodimeric LIF receptor complex has been shown to mediate the activities of oncostatin M (OSM), cardiotrophin-1 and ciliary neurotrophic factor (CNTF).

Human LIF R alpha  cDNA encodes a 1097 amino acid (aa) residue precursor type I membrane protein with a 44 aa residue signal peptide, a 789 aa residue extracellular domain, a 26 aa residue transmembrane domain, and a 238 aa residue cytoplasmic domain. LIF R alpha is a member of the cytokine receptor family and has extensive homology to gp130. The extracellular domain of LIF R alpha  has two cytokine receptor domains and three fibronectin type III repeats. In mouse, mRNAs encoding a soluble LIF R alpha and lacking transmembrane and intracellular domains, have been isolated. Soluble LIF R alpha has been shown to bind LIF and has LIF antagonistic activity.

  • References:
    1. Bazan, J.F. 1990, Proc. Natl. Acad. Sci. USA 87:6934.
    2. Gearing, D.P. (1994) Guidebook to Cytokines and Their Receptors, Academic Press, p130.
    3. Pennica D. et al. (1995) J. Biol. Chem. 270:10915.
  • Long Name:
    Leukemia Inhibitory Factor Receptor alpha
  • Entrez Gene IDs:
    3977 (Human); 16880 (Mouse); 81680 (Rat)
  • Alternate Names:
    CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R beta; LIF receptor; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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