Detects human ILT6/CD85e in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) ILT2, rhILT4, ILT5 is observed, and less than 5% cross-reactivity with rhLIR5 and rhLIR7 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human ILT6/CD85e Arg18-Glu439 Accession # Q8N6C8
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
Intracellular Staining by Flow Cytometry
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LILRA3/CD85e in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Goat Anti-Human LILRA3/CD85e Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2574G) or (B) Normal Goat IgG Alexa Fluor 488 Control (Catalog # IC108G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Human LIR-4 (Leukocyte Ig-like receptor #4; also ILT6 and LILRA3) is an Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LIR-4 is 416 amino acids in length and contains four C2-type Ig-like domains. Unlike other LIR family members, LIR-4 is actively secreted. At least one alternate splice form is known which shows a 64 amino acid deletion in the C-terminal half of the second Ig-like domain. Multiple alleles exist in human, and 5-10% of the population have a defective gene which produces no LIR-4 product. Rodent LIR-4 has not been reported.
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM and ITIM Domains), Member 3
Entrez Gene IDs:
CD85 antigen-like family member E; CD85e antigen; CD85e; HM31; HM43; ILT6; ILT-6; ILT6CD85e; Immunoglobulin-like transcript 6; leucocyte immunoglobulin-like receptor; Leukocyte immunoglobulin-like receptor 4; leukocyte immunoglobulin-like receptor A3; leukocyte immunoglobulin-like receptor subfamily A member 3; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member3; LIR4; LIR4CD85E; LIR-4HM43e3; Monocyte inhibitory receptor HM43/HM31
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