Human LIMPII/SR-B2 Antibody

Catalog # Availability Size / Price Qty
AF1966
AF1966-SP
Product Details
Citations (9)
FAQs
Supplemental Products
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Human LIMPII/SR-B2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIMPII in ELISAs and Western blots. In sandwich immunoassays, approximately 6% cross-reactivity with recombinant mouse LIMPII is observed and less than 0.3% cross-reactivity with recombinant human (rh) SR-B1 and rhCD36 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LIMPII lumenal loop
Arg27-Thr432
Accession # Q14108
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LIMPII/SR-B2 Fc Chimera (Catalog # 1966-LM)

Human LIMPII/SR-B2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human LIMPII/SR-B2 Lumenal Loop Biotinylated Antibody (Catalog # BAF1966)

Standard: Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF (Catalog # 1966-LM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIMPII/SR-B2

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and as CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1 - 3). SR-B/CD36 family members are type III integral membrane proteins with an N- as well as a C-terminal cytoplasmic tail, and a large extracellular (or lumenal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is widely expressed on all tissues and cell types so far examined. It is also expressed on the surface of activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII-thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation (1). Overexpression of LIMPII causes an enlargement of early endosomes and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

References
  1. Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855. 
  2. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
  3. Eskelinen, E-L. et al. (2003) Trends in Cell Biology 13:137.
  4. Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
  5. Gamp, A-C. et al. (2003) Human Molecular Genetics 12:631.
Long Name
Lysosomal Integral Membrane Protein II
Entrez Gene IDs
950 (Human); 12492 (Mouse)
Alternate Names
AMRF; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II); CD36 antigen; CD36L2; CD36L2LIMP II; HLGP85; LGP85; LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; LIMPII; LIMPIICD36 antigen-like 2; LPG85; lysosome membrane protein 2; Lysosome membrane protein II; SCARB2; Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein; scavenger receptor class B, member 2; SRB2; SR-B2; SR-BII

Product Datasheets

Citations for Human LIMPII/SR-B2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers
    Authors: GF Jang, JS Crabb, B Hu, B Willard, H Kalirai, AD Singh, SE Coupland, JW Crabb
    Cancers, 2021;13(14):.
    Species: Human
    Sample Types: Tissue Homogenates
  2. Involvement of VCP/UFD1/Nucleolin in the viral entry of Enterovirus A species
    Authors: J Yan, M Wang, M Wang, Y Dun, L Zhu, Z Yi, S Zhang
    Virus Res., 2020;283(0):197974.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Heparan sulfate attachment receptor is a major selection factor for attenuated enterovirus 71 mutants during cell culture adaptation
    Authors: K Kobayashi, K Mizuta, S Koike
    PLoS Pathog., 2020;16(3):e1008428.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Fibronectin facilitates EV71 infection by mediating its entry
    Authors: QQ He, S Ren, ZC Xia, ZK Cheng, NF Peng, Y Zhu
    J. Virol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. The binding of a monoclonal antibody to the apical region of SCARB2 blocks EV71 infection
    Authors: X Zhang, P Yang, N Wang, J Zhang, J Li, H Guo, X Yin, Z Rao, X Wang, L Zhang
    Protein Cell, 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Transcriptome analysis reveals dynamic changes in coxsackievirus A16 infected HEK 293T cells
    Authors: J Jin, R Li, C Jiang, R Zhang, X Ge, F Liang, X Sheng, W Dai, M Chen, J Wu, J Xiao, W Su
    BMC Genomics, 2017;18(0):933.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Amphotericin B Inhibits Enterovirus 71 Replication by Impeding Viral Entry
    Sci Rep, 2016;6(0):33150.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71.
    Authors: Leong, Shi Yun, Ong, Bryan Ki, Chu, Justin J
    PLoS Pathog, 2015;11(3):e1004686.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  9. Scavenger receptor B2 is a cellular receptor for enterovirus 71.
    Authors: Yamayoshi S, Yamashita Y, Li J, Hanagata N, Minowa T, Takemura T, Koike S
    Nat. Med., 2009;15(7):798-801.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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