LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells, and binds multiple ligands, including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development, and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905). In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 88% aa sequence identity with mouse LRP‑4.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 814334
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LRP-4
Ser21-Ser1725 (predicted)
Accession # O75096
Ser21-Ser1725 (predicted)
Accession # O75096
Specificity
Detects human LRP-4 in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human LRP‑4 Antibody
Detection of LRP‑4 in U‑87 MG Human Cell Line by Flow Cytometry.
U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human LRP-4 Monoclonal Antibody (Catalog # MAB59481, filled histo-gram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Human LRP‑4 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: U‑87 MG human glioblastoma/astrocytoma cell line
Sample: U‑87 MG human glioblastoma/astrocytoma cell line
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LRP-4
Long Name
LDL Receptor-related Protein 4
Alternate Names
LRP10, LRP4
Gene Symbol
LRP4
UniProt
Additional LRP-4 Products
Product Documents for Human LRP‑4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human LRP‑4 Antibody
For research use only
Related Research Areas
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