Human LRP-4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Rat LRP‑4 by Western Blot.
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Product Details
Supplemental Products

Human LRP-4 Antibody Summary

Species Reactivity
Detects human LRP-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) LRP-5 or rhLRP-6 is observed.
Monoclonal Mouse IgG1 Clone # 741704
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human LRP-4
Accession # O75096
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Rat LRP-4 antibody by Western Blot. View Larger

Detection of Human and Rat LRP‑4 by Western Blot. Western blot shows lysates of rat embryonic cortical glial cells and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LRP-4 Monoclonal Antibody (Catalog # MAB5948) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-4 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of LRP-4 antibody in U-2 OS Human Cell Line antibody by Flow Cytometry. View Larger

Detection of LRP‑4 in U-2 OS Human Cell Line by Flow Cytometry. U-2 OS human osteosarcoma cell line was stained with Mouse Anti-Human LRP-4 Monoclonal Antibody (Catalog # MAB5948, filled histo-gram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry LRP-4 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

LRP‑4 in Human Brain. LRP-4 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human LRP-4 Monoclonal Antibody (Catalog # MAB5948) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRP-4

LRP‑4 (Low density lipoprotein-related protein #4; also MEGF7 and LRP13) is a 220-270 kDa glycoprotein, member of the LDLR family of proteins. It is expressed on neurons, oocytes, spermatogonia and skeletal muscle cells and binds multiple ligands including WISE, apoE, MuSK and neuronal Agrin. It serves to negatively regulate Wnt signaling during development and to cluster AChRs at neuromuscular junctions. Mature human LRP‑4 is an 1885 amino acid (aa) type I transmembrane glycoprotein. It contains a 1705 aa extracellular domain (ECD) (aa 21-1725) plus a 159 aa cytoplasmic region (aa 1747-1905). In the ECD, there are eight LDLR class A repeats (aa 26-350), two EGF-like repeats (aa 354-434) and 20 LDLR class B repeats that contain an intervening EGF-like domain (aa 480-1610). Over aa 18-344, human LRP‑4 shares 97% aa identity with both mouse and rat LRP‑4. MAB5948 detects the correct rat band in Western Blot. Immunohistochemistry was not tested in rat samples.  

Long Name
LDL Receptor-related Protein 4
Entrez Gene IDs
4038 (Human); 228357 (Mouse); 83469 (Rat)
Alternate Names
CLSS; KIAA0816; low density lipoprotein receptor-related protein 4; low-density lipoprotein receptor-related protein 4; LRP10; LRP4; LRP-4; MEGF7LRP10; Multiple epidermal growth factor-like domains 7

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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