Human LRRTM1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LRRTM1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross reactivity with recombinant human (rh) LRRTM2 is observed, and less than 5% cross reactivity rhLRRTM3 and rhLRRTM4 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LRRTM1
    Ala35-Ile425
    Accession # Q86UE6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human LRRTM1 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human LRRTM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4897) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRRTM1

Human LRRTM1 (leucine-rich repeat transmembrane neuronal 1) is a 58‑59 kDa (predicted) type I transmembrane protein, that belongs to the LRRTM family of proteins within the leucine-rich repeat (LRR) superfamily (1). It is synthesized as a precursor with a 34 amino acid (aa) signal sequence, a 393 aa luminal region, a 21 aa transmembrane region, and a 74 aa cytoplasmic region. The luminal portion of LRRTM1 contains three N-linked glycosylation sites and 10 LRRs flanked by cysteine-rich domains (1). The cytoplasmic region contains several tyrosine, serine, and threonine residues that have potential to be phosphorylated and thus to be involved in signal transduction (1). The C-terminal also contains a conserved glutamic acid-cysteine-glutamic acid-valine sequence for potential interaction with PDZ proteins (1‑2). Mature human LRRTM1 is 97% aa identical to mouse LRRTM1. LRRTM1 is localized to the endoplasmic reticulum (3). In the mouse, beginning at 9dpc, low levels of LRRTM1 can be detected in the overlying ectoderm of the limb bud, dorsal otic vesicle, forebrain, midbrain, hindbrain, and in neural progenitors in the central neural tube (2). In the adult brain, it is highly expressed in the brain and salivary gland, and is detected at intermediate levels in the cerebellum, spinal chord, stomach, testis, and uterus (1). Functionally, LRRTM1 may be involved in the formation of the CNS and maintenance of CNS structure and function in the adult brain (1). In addition, LRRTM1 has been shown to be a maternally suppressed gene that is associated paternally with handedness and schizophrenia (3).

  • References:
    1. Lauren, J. et al. (2003) Genomics 81:411.
    2. Haines, B.P. and P.W.J. Rigby (2007) Gene Expr. Patterns 7:23.
    3. Francks, C. et al. (2007) Mol. Psychiatry 12:1129.
  • Long Name:
    Leucine Rich Repeat Transmembrane Neuronal 1
  • Entrez Gene IDs:
    347730 (Human); 74342 (Mouse); 679668 (Rat)
  • Alternate Names:
    FLJ32082; leucine rich repeat transmembrane neuronal 1; leucine-rich repeat transmembrane neuronal 1 protein; leucine-rich repeat transmembrane neuronal protein 1; LRRTM1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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