|Detection of Human LTK by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human LTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LTK at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
|LTK in Human Brain. LTK was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4664) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
LTK (Leukocyte tyrosine kinase; also protein tyrosine kinase 1) is a glycoprotein member of the tyrosine protein kinase family, insulin receptor subfamily of proteins. There are multiple isoforms that run between 50 and 100 kDa in SDS-Page. It is reportedly expressed in lymphocytes and cerebral cortex neurons, plus cardiomyocytes where it plays a role in cellular hypertrophy. Mature human LTK is an 848 amino acid (aa) type I transmembrane protein that is embedded in the ER. It contains a 408 aa extracellular domain (aa 17-424) plus a 415 aa cytoplasmic region (aa 450-864). The cytoplasmic region possesses multiple phosphotyrosines that interact with downstream signaling molecules. There is also a protein kinase domain between aa 510-786. LTK apparently undergoes dimer- and trimerization under certain circumstances. There are multiple splice variants for LTK. Two isoforms possess a common deletion of aa 274-334 that may be accompanied by a 27 aa substitution for aa 449-544. Another isoform shows an alternative start site at Met406 accompanied by a 17 aa substitution for Val449, while a fourth isoform contains a 51 aa substitution for aa 171-864. A final 50 kDa isoform shows a 28 aa substitution for aa 449-864. Over aa 17-424, human LTK shares 75% aa identity with mouse LTK.
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