Human LTK Antibody

Catalog # Availability Size / Price Qty
AF4664
AF4664-SP
Human LTK Antibody in Western Blot
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Human LTK Antibody Summary

Species Reactivity
Human
Specificity
Detects human LTK in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LTK
Ser10-Pro424
Accession # P29376
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human LTK by Western Blot. View Larger

Detection of Human LTK by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human LTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LTK at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry LTK in Human Brain. View Larger

LTK in Human Brain. LTK was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4664) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LTK

LTK (Leukocyte tyrosine kinase; also protein tyrosine kinase 1) is a glycoprotein member of the tyrosine protein kinase family, insulin receptor subfamily of proteins. There are multiple isoforms that run between 50 and 100 kDa in SDS-Page. It is reportedly expressed in lymphocytes and cerebral cortex neurons, plus cardiomyocytes where it plays a role in cellular hypertrophy. Mature human LTK is an 848 amino acid (aa) type I transmembrane protein that is embedded in the ER. It contains a 408 aa extracellular domain (aa 17-424) plus a 415 aa cytoplasmic region (aa 450-864). The cytoplasmic region possesses multiple phosphotyrosines that interact with downstream signaling molecules. There is also a protein kinase domain between aa 510-786. LTK apparently undergoes dimer- and trimerization under certain circumstances. There are multiple splice variants for LTK. Two isoforms possess a common deletion of aa 274-334 that may be accompanied by a 27 aa substitution for aa 449-544. Another isoform shows an alternative start site at Met406 accompanied by a 17 aa substitution for Val449, while a fourth isoform contains a 51 aa substitution for aa 171-864. A final 50 kDa isoform shows a 28 aa substitution for aa 449-864. Over aa 17-424, human LTK shares 75% aa identity with mouse LTK.

Long Name
Leukocyte Tyrosine Kinase Receptor
Entrez Gene IDs
4058 (Human); 17005 (Mouse); 311337 (Rat)
Alternate Names
EC 2.7.10.1; leukocyte receptor tyrosine kinase; leukocyte tyrosine kinase receptor; leukocyte tyrosine kinase; LTK; TYK1; TYK1Protein tyrosine kinase 1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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