Human mGluR4 Antibody
Human mGluR4 Antibody Summary
Accession # Q14833
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of mGluR4 in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Sheep Anti-Human mGluR4 Affinity-purified Polyclonal Antibody (Catalog # AF7387, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).
mGluR4 in Human Endothelial Cells. mGluR4 was detected in immersion fixed paraffin-embedded sections of human brainstem (medulla) using Sheep Anti-Human mGluR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7387) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Metabotrophic glutamate receptor 4 (mGluR4) is a 95-110 kDa Group III mGluR member of the Class C, GPCR 3 family of proteins. The mGluR family of receptors are 7-transmembrane (TM), non-ion channel glutamate-binding molecules that are linked to adenylcyclase. mGluR4 is widely expressed, being found on/in multiple cell types, including colonic, breast, urinary and skin epithelium. It is also found on neurons where it is located presynaptically and regulates neurotransmitter release. In general, mGluR4 activation results in a slowing of either glutamine or GABA release. However, in discrete areas, it actually facilitates glutamine release. Mature human mGluR4 is an 880 amino acid (aa) 7-TM molecule (aa 33-912). It contains a 555 aa N-terminal extracellular region (aa 33-587), plus a 65 aa C-terminal cytoplasmic domain. mGluR4 potentially forms homodimers, and is reported to heterodimerize with both mGluR2 and mGluR8. Based on rodent, there is one 68 kDa isoform that is found in taste buds and shows an alternative start site at Met309. Two additional potential splice variants contain a second alternative start site at Met209, and an Asp substitution for aa 343-390, respectively. It is uncertain if human possesses a mGluR4b found in rodent. Over aa 1-518, human mGluR4 shares 96% aa identity with mouse mGluR4.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human mGluR4 Antibody
There are currently no reviews for this product. Be the first to review Human mGluR4 Antibody and earn rewards!
Have you used Human mGluR4 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image