Human MICL/CLEC12A Antibody

Catalog # Availability Size / Price Qty
AF2946
AF2946-SP
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Human MICL/CLEC12A Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICL/CLEC12A in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant mouse MICL is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICL/CLEC12A isoform a
Thr67-Ala265
Accession # NP_612210
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MICL/CLEC12A
Immunocytochemistry
5-15 µg/mL
Immersion fixed activated human peripheral blood mononuclear cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICL/CLEC12A

MICL, also known as C-type lectin-like molecule-1 (CLL-1), Killer cell lectin-like receptor KLRL1 and C type lectin domain family 12, member A (CLEC12A), is a type II transmembrane highly glycosylated protein that contains a functional tyrosine-based inhibitory motif in its cytoplasmic tail. The gene for MICL has been mapped to the natural killer gene complex (NKC) on human chromosome 12p13.2. MICL contains a non-classic C-type domain that binds non-sugar ligands. It is primarily expressed on NK cells, T cells, dendritic cells, monocytes and macrophages. By alternative splicing, multiple splice isoforms exist. MICL is a negative regulator of granulocytes and monocyte function and inhibits NK cell cytotoxicity. The amino acid sequence of human MICL extracellular domain is 53%, 65% and 55% identical to that of mouse, canine and bovine MICL, respectively.

Long Name
Myeloid Inhibitory C-type Lectin-like Receptor
Entrez Gene IDs
160364 (Human); 232413 (Mouse)
Alternate Names
CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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