Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Detection

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all MMP-13 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-13 (R&D Systems, Catalog # 511-MM)
Leu20-Cys471
Accession # P45452

Specificity

Detects human MMP-13 in Western blots. In this format, approximately 5% cross-reactivity with recombinant human (rh) MMP‑8 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -9, and -12 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human MMP‑13 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human MMP‑13 (Catalog # 511-MM)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MMP-13

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is distinguished from the other human collagenases by its effecient degradation of type II collagen. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. Structurally, MMP-13 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References

  1. Jeffery, J.J. (1998) in Collagenase 3. A.J. Barrett, et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1167.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Entrez Gene IDs

4322 (Human)

Gene Symbol

MMP13

UniProt

P45452

Additional MMP-13 Products

Product Documents for Human MMP‑13 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MMP‑13 Biotinylated Antibody

For research use only

Citations for Human MMP‑13 Biotinylated Antibody

Customer Reviews for Human MMP‑13 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human MMP‑13 Biotinylated Antibody and earn rewards!

Have you used Human MMP‑13 Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies