Human/Mouse ADAMTS15 Antibody

R&D Systems | Catalog # AF5149

R&D Systems
Discontinued Product
AF5149 has been discontinued. View all ADAMTS15 products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ADAMTS15 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS15
Gly18-Cys682
Accession # Q8TE58

Specificity

Detects human and mouse ADAMTS15 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human ADAMTS1, L1.2, L2, 3, 4, 5, 8, 10, 12, 13, and 16 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse ADAMTS15 Antibody

Detection of Human and Mouse ADAMTS15 antibody by Western Blot.

Detection of Human and Mouse ADAMTS15 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, BG01V human embryonic stem cells, and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse ADAMTS15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ADAMTS15 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

ADAMTS15 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

ADAMTS15 in Human Breast Cancer Tissue.

ADAMTS15 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human/Mouse ADAMTS15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5149) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse ADAMTS15 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS15 (Catalog # 5149-AD), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line, BG01V human embryonic stem cells, and mouse embryo tissue

Reviewed Applications

Read 1 review rated 4 using AF5149 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ADAMTS15

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) is a group of nineteen secreted zinc binding metalloproteases that is involved in development, inflammation, angiogenesis and several pathological conditions (1, 2). They are synthesized as zymogens which have a pro-domain that is removed by furin-like protein convertases. The mature secreted enzymes consist of catalytic, disintegrin-like, type I thrombospondin (TSP) motif, cysteine-rich, and spacer domains followed by zero to fourteen additional TSP motifs. Among ADAMTS proteases, ADAMTS1, -4, -5, -8, and -15 form a subfamily that possess aggrecanase activity (1 - 3). While ADAMTS4 and -5 (also known as aggrecanse-1 and -2, respectively) (4) have been well studied, the enzymatic activity of ADAMTS15 remains to be explored. It is suggested that ADAMTS15 may be involved in aggrecan degradation and breast cancer (5, 6). ADAMTS15 may also exhibit tumor suppressor activities since it contains genetic mutations in human colorectal carcinomas and restrains tumor growth and invasion in both in vitro and in vivo studies (7). The purified rhADAMTS15 is truncated and ends before the spacer domain.

References

  1. Porter, S. et al. (2005) Biochem. J. 386:15.
  2. Jones, G.C. and G.P. Riley (2005) Arthritis Res. Ther. 7:160.
  3. Nicholson, A.C. et al. (2005) BMC Evol. Biol. 5:11.
  4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  5. Porter, S. et al. (2006) Intl. J. Cancer 118:1241.
  6. Kevorkian, L. et al. (2004) Arthritis Rheum. 50:131.
  7. Viloria, C.G. et al. (2009) Cancer Res. 69:4926.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 15

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, preproprotein, ADAM metallopeptidase with thrombospondin type 1 motif, 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15, EC 3.4.24, EC 3.4.24.-, EC 3.4.24.14, EC 3.4.24.82, metalloprotease disintegrin 15 with thrombospondin domains, MGC126403

Entrez Gene IDs

170689 (Human); 235130 (Mouse); 300474 (Rat)

Gene Symbol

ADAMTS15

UniProt

Q8TE58

Additional ADAMTS15 Products

Product Documents for Human/Mouse ADAMTS15 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse ADAMTS15 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Human/Mouse ADAMTS15 Antibody (1)

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  • Human/Mouse ADAMTS15 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/04/2019
    We used this antibody for developing a sandwich ELISA in combination with mAb (5149) and Protein std (5149-AD). This combination worked well and detected ADAMTD 15 efficiently in human serum and plasma samples.
    Human/Mouse ADAMTS15 Antibody AF5149

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