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Product Details
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Reviews
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Species ReactivityHuman, Mouse
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SpecificityDetects human alpha -Fetoprotein/AFP in direct ELISAs and Western blots.
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SourceMonoclonal Mouse IgG1 Clone # 189502
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PurificationProtein A or G purified from hybridoma culture supernatant
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ImmunogenHuman umbilical cord serum-derived alpha -Fetoprotein/AFP
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FormulationSupplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
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LabelAlexa Fluor 405
Product Datasheets
Certificate of Analysis Lookup
Certificate of Analysis Lookup
Certificate of Analysis Request Form
Applications
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Recommended
ConcentrationSample
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Intracellular Staining by Flow Cytometry0.25-1 µg/106 cellsSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application.
General Protocols
are available in the Technical Information section on our website.
Data Example
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Intracellular Staining by Flow Cytometry
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Detection of a-Fetoprotein/AFP in Human HepG2Cell Line by Flow Cytometry. HepG2 Human hepatocellular carcinomacell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP Alexa Fluor® 405-conjugatedMonoclonal Antibody (Catalog # IC1368V, filled histogram) or isotype controlantibody (Catalog # IC002V, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules. |
Preparation and Storage
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ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
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Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 degreesC as supplied.
Background: alpha-Fetoprotein/AFP
alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.
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Entrez Gene IDs:174 (Human); 11576 (Mouse); 24177 (Rat)
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Alternate Names:AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP
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