Human/Mouse alpha -Fetoprotein/AFP Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1368V-100UG
Detection of a-Fetoprotein/AFP in Human HepG2Cell Line by Flow Cytometry.
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Human/Mouse alpha -Fetoprotein/AFP Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 189502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of a-Fetoprotein/AFP in Human HepG2Cell Line by Flow Cytometry. View Larger

Detection of a-Fetoprotein/AFP in Human HepG2Cell Line by Flow Cytometry. HepG2 Human hepatocellular carcinomacell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP Alexa Fluor® 405-conjugatedMonoclonal Antibody (Catalog # IC1368V, filled histogram) or isotype controlantibody (Catalog # IC002V, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Fetoprotein/AFP

alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.

Entrez Gene IDs
174 (Human); 11576 (Mouse); 24177 (Rat)
Alternate Names
AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP

Product Datasheets

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