Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP PE-conjugated Monoclonal Antibody (Catalog # IC1368P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
alpha -Fetoprotein/AFP Specificity is Shown by Flow Cytometry in Knockout Cell Line. a-Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with PE-conjugated Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # IC1368P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the a-Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.
Citations for Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Generation of iPSCs from Jaw Periosteal Cells Using Self-Replicating RNA
Authors: F Umrath, H Steinle, M Weber, HP Wendel, S Reinert, D Alexander, M Avci-Adali
Int J Mol Sci, 2019;20(7):.
Sample Types: Whole Cells
Applications: Flow Cytometry
Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA
Authors: H Steinle, M Weber, A Behring, U Mau-Holzma, C Schlensak, HP Wendel, M Avci-Adali
Stem Cells Int, 2019;2019(0):7641767.
Applications: Flow Cytometry
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