Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Knockout Validated, Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 189502
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP

Specificity

Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Antibody

Detection of a-Fetoprotein/AFP antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP PE-conjugated Monoclonal Antibody (Catalog # IC1368P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
a-Fetoprotein/AFP Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

alpha -Fetoprotein/AFP Specificity is Shown by Flow Cytometry in Knockout Cell Line.

a-Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with PE-conjugated Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # IC1368P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the a-Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Knockout Validated

alpha -Fetoprotein/AFP is specifically detected in HepG2 hepatocellular carcinoma parental cell line but is not detectable in alpha -Fetoprotein/AFP knockout HepG2 cell line.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: alpha-Fetoprotein/AFP

alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Antibody

For research use only

Citations for Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Antibody

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